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首页> 外文期刊>Tropical Animal Health and Production >High frequency of seropositive and carriers of Leptospira spp. in pigs in the semiarid region of northeastern Brazil
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High frequency of seropositive and carriers of Leptospira spp. in pigs in the semiarid region of northeastern Brazil

机译：荔枝杆菌SPP的血清阳性和载体高频。 在巴西东北部的半干旱地区的猪

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摘要

Carrier animals are considered key in the transmission cycle of leptospirosis. Although investigations have been carried out on several species, the role of pigs in the epidemiology of the disease is still poorly studied in the semi-arid region. Thus, the objective of this study was to determine the presence of Leptospira spp. in the genitourinary tract of pigs intended for slaughter. Fifty pigs were used: adults and unvaccinated. Samples of the kidney, urine, and vaginal fluid were collected for the molecular detection of Leptospira spp. and blood samples for the serological test. The molecular test was performed using the polymerase chain reaction (PCR), and the serological test was performed with the microscopic agglutination test (MAT). Samples with DNA amplification were submitted to genetic sequencing. Twenty (40%) animals were found with anti-Leptospira spp. antibodies, and the majority of the reactions (50%) occurred for the serogroup Tarassovi. Leptospiral DNA was found in the tissue of 11 (22%) pigs. The gene from a urine sample was sequenced and showed similarity to L. borgpetersenii. The results evidenced a high rate of porcine carriers; therefore, they appear to be important sources of agent infection, being potential transmitters of the disease to other animal species and man.
机译：载体动物被认为是钩端螺旋体病的传输循环中的关键。虽然对几种物种进行了调查，但在半干旱地区仍然在疾病流行病学中的作用仍然很差。因此，本研究的目的是确定乳化物SPP的存在。在用于屠宰的猪的泌尿生殖道中。使用了五十只猪：成年人和未被解雇。收集肾脏，尿液和阴道液的样品，用于leptospira spp的分子检测。和血液样品用于血清学测试。使用聚合酶链反应（PCR）进行分子试验，并用微观凝集试验（垫）进行血清学试验。具有DNA扩增的样品被提交至遗传测序。用抗睑板SPP发现二十（40％）动物。抗体和大部分反应（50％）发生血清毒素氧化术。在11（22％）猪的组织中发现了瘦身血液DNA。将来自尿液样品的基因测序并显示出与L. borgpetersenii相似之处。结果证明了猪携带者的高速率;因此，它们似乎是代理感染的重要来源，是疾病的潜在发射器到其他动物物种和人物。

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