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首页> 外文期刊>Tropical Animal Health and Production >Polymorphism of lactoferrin gene in Egyptian goats and its association with milk composition traits in Zaraibi breed
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Polymorphism of lactoferrin gene in Egyptian goats and its association with milk composition traits in Zaraibi breed

机译：埃及山羊乳蛋白基因的多态性及其与Zaraibi品种牛奶组合物性状的关系

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摘要

The objectives of this study were to identify polymorphisms in the lactoferrin gene among three Egyptian goat breeds (Barki, Zaraibi, and Damascus) and to investigate the effect of LF genotype, parity, and lactation stage on milk composition traits of Zaraibi goats. One hundred and thirty-two blood samples were collected for DNA extraction, with 53 from Zaraibi, 40 from Damascus, and 39 from Barki breeds. Fat, protein, total solids, solids-not-fat, and lactose percentages were determined in Zaraibi goat milk using an automatic milk analyzer. Two genotypes, GG and GA, in the lactoferrin gene were identified using single-strand conformation polymorphism and were confirmed by direct sequencing technique. The Zaraibi breed recorded the highest heterozygosity (0.272) and effective number of alleles (1.369), while the Damascus breed recorded the lowest values. The G/A SNP showed a significant association with protein, solids-not-fat, and total solid content of Zaraibi goat milk. Protein, solids-not-fat, and total solid content in our study were significantly higher at early and late parities. Lactose percentage decreased significantly from early to late parity. Fat, protein, solids-not-fat, and total solid content were significantly higher at early and late stages of lactation, and our results encourage the utilization of Zaraibi goat milk in cheese and butter processing at these stages. Moreover, the G/A SNP of the LF gene may be a useful marker for assisted selection programs to improve goat milk composition.
机译：本研究的目标是鉴定三埃山羊品种（Barki，Zaraibi和大马士革）中乳铁蛋白基因的多态性，并研究LF基因型，平价和哺乳期对Zaraibi山羊牛奶组合物性状的影响。收集了一百三十两种血液样品，用于DNA提取，Zaraibi，来自大马士革40的53，来自巴西品种39种。使用自动牛奶分析仪在Zaraibi山羊牛奶中测定脂肪，蛋白质，总固体，固体 - 不脂肪和乳糖百分比。使用单链构象多态性鉴定了在乳铁蛋白基因中的两个基因型，GG和Ga，并通过直接测序技术证实。 Zaraibi品种记录了最高的杂合子（0.272）和有效数量的等位基因（1.369），而大马士革品种记录了最低值。 G / A SNP显示出与蛋白质，固体 - 不脂肪和Zaraibi山羊牛奶的总固体含量有显着关系。在早期和晚期，我们研究中的蛋白质，固体 - 不脂肪和全固体含量明显高度较高。乳糖百分比从早期到晚期均显着下降。在哺乳期的早期和晚期和晚期阶段的脂肪，蛋白质，固体 - 不脂肪和总固体含量显着提高，我们的结果促进了这些阶段的奶酪和黄油加工中的Zaraibi山羊牛奶。此外，LF基因的G / A SNP可以是用于辅助选择程序以改善山羊牛奶组合物的有用标记。

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