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首页> 外文期刊>Tropical Animal Health and Production >DiarrheagenicEscherichia coliisolates from neonatal alpacas mainly display F17 fimbriae adhesion gene
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DiarrheagenicEscherichia coliisolates from neonatal alpacas mainly display F17 fimbriae adhesion gene

机译：Nearrheagenicescherichia来自新生儿羊驼的Coliisolates主要显示F17 Fimbriae粘附基因

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摘要

Neonatal domestic South American Camelid llamas and alpacas suffer from an enteric disease complex characterized by abdominal distention, lethargy, dehydration, and eventual fatal septicemia. Analysis of rectal swabs from neonatal alpacas suffering clinical diarrheas has constantly isolatedEscherichia coli, mainly the EPEC and EHEC pathotypes. The present communication reports the results of molecular analysis of 226E. colistrains from neonatal alpaca rectal swabs. The isolates were initially tested by multiple PCR, to identifyE. colivirulence geneseae,bfp,Lt,Stx1,Stx2,sta,stab, andltgenes and a similar test to detect F4, F5, F6, F17, and F41fimbriae adhesin genes. Forty-two of the 226 (18.5%) isolates tested positive for at least one pathogenic gene, 25 of the 42 were classified as EPEC (3 positives for onlyeaeand 22 for botheaeandbfp) and the remaining 17 were classified as EHEC. Twenty-four (57%) of the 42 isolates tested positive to F17 adhesin while one was positive for both F6 and F17.
机译：新生儿国内南美骆驼骆驼和羊驼患有肠道疾病复合体，其特征在于腹胀，嗜睡，脱水，最终致命的败血症。患有临床腹泻的新生儿Alpacas的直肠拭子分析持续分离出Coli，主要是EPEC和EHEC病理型。本通讯报告了226E的分子分析结果。来自新生儿羊驼直肠拭子的Colistrains。分离株最初通过多种PCR测试以鉴定。 CoIvirululence eneeae，BFP，LT，STX1，STX2，STA，刺，ANDLTGENE和类似的测试，以检测F4，F5，F6，F17和F41FIMBRIAE粘附基因。 226（18.5％）分离物中的426（18.5％）的分离物用于至少一种致病基因的阳性，42个中的25例被归类为EPEC（仅适用于两种22的3个阳性），其余17分为eHEC。二十四（57％）的42个分离物测试阳性至F17粘附蛋白，而一种F6和F17均为阳性。

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