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首页> 外文期刊>Tropical Animal Health and Production >Association of allelic polymorphisms of IGFALS gene with growth traits in Makouei and Ghezel sheep breeds
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Association of allelic polymorphisms of IGFALS gene with growth traits in Makouei and Ghezel sheep breeds

机译：IGFALS基因的等位基因多态性与Makouei和Ghezel绵羊繁殖的生长性状

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摘要

Insulin-like growth factor-binding protein acid-labile subunit (IGFALS) encodes a protein which binds to IGF1 and IGFBP-3 to regulate the growth, differentiation, and other physiological processes. The aim of this study was the identification of allelic polymorphisms of the IGFALS gene using the PCR-RFLP technique and evaluation of their association with growth traits. For this end, 120 blood samples were randomly collected from each breed. Following amplification of an 1113-bp fragment of exon 1 and a part of intron 1 of the IGFLAS gene, genotyping was conducted by three restriction enzymes includingHinfI,MscI, andPvuII. The results showed that only one allele was observed in IGFALS-PvuIIsite, while in IGFLAS-MscIsite, three AA, AB, and BB genotypes with the frequencies of 17.5%, 32%, and 50.5% and 11%, 37.5%, and 51.5% were observed in Makouei and Ghezel sheep breeds, respectively. Additionally, in the IGFLAS-HinfIsite, two AB and BB genotypes with the frequency of 34.2% and 65.8% were observed in Makouei sheep and AA, AB, and BB genotypes with the frequency of 9%, 21%, and 70% were observed in Ghezel sheep. So that, Makouei sheep with AB genotype had more chest girth (CG) compared with other genotypes. Furthermore, a significant association was observed between the genotypes of IGFLAS-HinfIwith birth weight (BW) in Ghezel and BW, weaning weight (BW3), and CG in Makouei sheep. Haplotype analysis revealed an association between paternal haplotypes and BW in both Ghezel and Makouei breeds. So that, AAB and ABB haplotypes showed more BW than others in Makouei and Ghezel sheep, respectively.
机译：胰岛素样生长因子结合蛋白质酸不稳定亚基（IgFals）编码与IGF1和IGFBP-3结合的蛋白质，以调节生长，分化和其他生理过程。本研究的目的是使用PCR-RFLP技术鉴定IGFALS基因的等位基因多态性，并与其与生长特征的关系评估。为此，从每个品种随机收集120个血样。扩增EXON 1的1113bp片段和IGFLAS基因的内含子1的一部分后，基因分型通过三个限制酶进行，包括HINFI，MSCI，ANDPVUII。结果表明，在IGFALS-PVUIIIE中只观察到一个等位基因，而在IGFLAS-MSCISITE，三个AA，AB和BB基因型中，频率为17.5％，32％和50.5％和11％，37.5％和51.5 ％分别观察到Makouei和Ghezel绵羊品种。另外，在Igflas-hinfisite中，在Makouei绵羊和AA，AB和AA，AB和AA，AB和BB基因型中观察到频率为34.2％和65.8％的BB基因型，观察到9％，21％和70％的BB基因型在Ghezel羊。因此，与AB基因型的Makouei绵羊与其他基因型相比具有更多的胸腺周长（CG）。此外，在Gheizel和BW，断奶重量（BW3）和Makouei绵羊中的Igflas-hinfiwith的出生体重（BW）的基因型之间观察到显着关联。单倍型分析显示了Ghezel和Makouei品种的Paternal Haplotypes和BW之间的关联。因此，AAB和ABB单倍型分别显示出比Makouei和Ghezel Sheep中的其他人更多的BW。

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