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首页> 外文期刊>Tropical Animal Health and Production >Molecular and phenotypic characterization of enterococci isolated from broiler flocks in Turkey
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Molecular and phenotypic characterization of enterococci isolated from broiler flocks in Turkey

机译：土耳其肉鸡群中肠球菌分子和表型表征

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The aim of this study was to determine the antimicrobial resistance, resistance mechanisms implicated, and virulence genes (asa 1 gelE, cylA, esp, and hyl) of Enierococcus spp. isolated from broiler flocks in Turkey. In addition, clonality of ampicillin and vancomycin-resistant enterococci was also investigated using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Out of 430 cloacal swab samples investigated, 336 (78.1%) Enterucoccus spp. was isolated. The most frequently identified species were E. faecalis (87.8c%), E. faecium (8.3%), E. durans (2.4%), E. casseldlavus (0.9%), and E. hirae (0.6%). The most common resistance was against tetracycline (81.30/c), erythromycin (77.1%), ciprofloxacin (56.8%), and chloramphenicol (46.4%). Fifty (14.9c%) isolates showed high-level gentamicin resistance (HLGL) phenotype. Ampicillin and vancomycin resistance were observed in 3.30/c and 1.5% of the isolates, respectively. Two hundred eighty-three isolates were positive for the presence of virulence genes. Among the virulence genes tested, only gelE, asal, esp, and cylA genes were detected. The most prevalent virulence gene was gelE (234, 69.6%), followed by asal (160, 47.6%), esp (37, 11%), and cylA (2, 0.6%). In conclusion, this study revealed that commensal enterococci from broiler flocks showed high rate of resistance to antimicrobials including clinically important antimicrobials for humans. The main underlying reason for high resistance could be attributed to the inappropriate and widespread use of antimicrobials. Therefore, there is an urgent need to develop control strategies to prevent the emergence and spread of antimicrobial resistance.
机译：本研究的目的是确定抗微生物抗性，抗性机制的含有肠球菌的抗微生物抗性，抗性机制（ASA 1凝胶，ZEL，ESP和HYL）。从肉鸡的隔绝在土耳其。此外，还使用脉冲场凝胶电泳（PFGE）和多层序列键入（MLST）研究了氨苄青霉素和抗性肠肠肠细胞的克隆性。 430个核糖棉签样品中调查，336（78.1％）肠杆菌SPP。被孤立。最常鉴定的物种是E.粪便（87.8℃％），E.粪便（8.3％），E.杜兰斯（2.4％），E.CasseldLavus（0.9％）和E.Hirae（0.6％）。最常见的抗性是抗四环素（81.30 / c），红霉素（77.1％），环丙沙星（56.8％）和氯霉素（46.4％）。五十（14.9℃）分离物显示出高水平的庆大霉素抵抗（HLGL）表型。氨苄青霉素和万古霉素抗性分别在3.30 / c和1.5％的分离物中观察到。对于毒力基因存在，两百八十三个分离株是阳性的。在测试的毒力基因中，检测到仅凝胶，ASAL，ESP和曲柱基因。最普遍的毒力基因是凝胶（234,69.6％），其次是ASAL（160,47.6％），ESP（37,11％）和圆柱（2,0.6％）。总之，本研究表明，来自肉鸡羊群的共生肠球菌对抗微生物的耐受性高，包括用于人类的临床重要的抗微生物剂。高抗性的主要潜在原因可能归因于抗微生物的不恰当和广泛使用。因此，迫切需要制定控制策略以防止抗菌性抗性的出现和传播。

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