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首页> 外文期刊>Tropical Animal Health and Production >Comparison of rapid diagnostic tests to detect Mycobacterium avium subsp paratuberculosis disseminated infection in bovine liver
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Comparison of rapid diagnostic tests to detect Mycobacterium avium subsp paratuberculosis disseminated infection in bovine liver

机译：快速诊断试验检测牛肝脏分枝杆菌患者分枝杆菌患者的比较

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摘要

Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne's disease, a chronic enteritis in cattle and other domestic and wild ruminants. The presence of MAP in tissues other than intestines and associated lymph nodes, such as meat and liver, is a potential public health concern. In the present study, the relationship between the results of rapid diagnostic tests of the Johne's disease, such as serum ELISA, rectal scraping PCR, and acid-fast staining, and the presence of MAP in liver was evaluated. Blood, liver, and rectal scraping samples were collected from 200 slaughtered cattle with unknown Johne's disease status. ELISA was performed to determine the MAP antibody activity in the serum. Acid-fast staining was performed on rectal scraping samples, and PCR was performed on rectal scraping and liver samples. PCR-positive liver samples were used for mycobacterial culture. Overall, the results of this study demonstrated that MAP can be detected and cultured from liver of slaughtered cattle and rapid diagnostic tests of Johne's disease have limited value in detecting cattle with MAP infection in liver. These findings show that the presence of MAP in liver tissue may occur in cows with negative results for rapid diagnostic tests and vice versa. Hence, liver might represent another possible risk of human exposure to MAP. Given concerns about a potential zoonotic role for MAP, these results show the necessity to find new methods for detecting cattle with MAP disseminated infection.
机译：分枝杆菌亚空间。 paratuberculosis（地图）导致约翰的疾病，牛和其他国内和野生反刍动物的慢性肠炎。除肠和相关淋巴结之外的组织中的地图存在，如肉类和肝脏，是潜在的公共卫生问题。在本研究中，评估了Johne疾病的快速诊断试验结果的关系，例如血清ELISA，直肠刮削PCR和酸快染色以及肝脏中图的存在。从200个屠宰的牛群中收集血液，肝脏和直肠刮样品，含有未知的约翰疾病状态。进行ELISA以确定血清中的地图抗体活性。在直肠刮擦样品上进行酸快染色，并在直肠刮擦和肝脏样品上进行PCR。 PCR阳性肝脏样品用于分枝杆菌培养物。总体而言，本研究的结果表明，可以从屠宰牛的肝脏肝脏检测和培养地图，并在肝脏中检测牛的牛感染有限。这些发现表明，肝组织中的MAP的存在可能在奶牛中出现具有快速诊断测试的负面结果的奶牛，反之亦然。因此，肝脏可能代表人类暴露于地图的另一个可能风险。鉴于关于地图的潜在的动物区作用的担忧，这些结果表明了寻找用地图传播感染检测牛的新方法的必要性。

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