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首页> 外文期刊>Tropical Animal Health and Production >Antimicrobial resistance and virulence genes in theEscherichia coliisolates obtained from ostrich
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Antimicrobial resistance and virulence genes in theEscherichia coliisolates obtained from ostrich

机译：从鸵鸟获得的螺旋晶素抗菌和毒力基因

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摘要

Escherichia coli(E.coli) is a normal intestinal microflora of birds, including ostrich. However, some strains are pathogenic to ostrich. There is a lack of knowledge concerning the differences between commensal and pathogenicE.coliisolates of ostrich. This research aimed to characterize the antimicrobial susceptibility profile and virulence gene content of presumptive commensal and pathogenicE.coliisolates of ostrich. A total of 129 bacterial isolates (commensal strains no. = 45; pathogenic strains no. = 84) were obtained from ostriches. The resistance profile of these isolates was investigated by the disk diffusion method and PCR. Also, the strains were screened for virulence-associated genes of avian pathogenicE.coli(APEC). The study showed that the highest and the lowest antimicrobial resistance were against oxytetracycline and gentamicin, respectively. Oxytetracycline, florfenicol, and streptomycin resistance rate in pathogenic isolates were higher than commensal ones (p < 0.05). Also,tetA,blaTEM, andaac(3)-IVresistance genes were more prevalent in pathogenic than commensal isolates (p < 0.05). More than half of the isolates had no virulence-associated genes. The multiplex PCR results showed thatirp2gene was more prevalent in pathogenic than commensalE.coli(p < 0.05). Nevertheless this was not the case with the other genes. Our results indicated a low frequency of antimicrobial resistance and virulence genes inE.coliisolates of ostriches. Antimicrobial susceptibility profile and virulence gene content ofE.coliisolates of ostriches differ between presumptive commensal and pathogenic strains. However, more analyses are needed to discriminate these isolates.
机译：大肠杆菌（大肠杆菌）是鸟类的正常肠道微生物，包括鸵鸟。然而，一些菌株对鸵鸟来说是致病性。缺乏关于鸵鸟的共生和病因的差异的知识。鸵鸟的科学。该研究旨在表征推定的共生和病因的抗微生物敏感性曲线和毒力基因含量。鸵鸟的分离。总共129个细菌分离株（非团结菌株。= 45;从鸵鸟获得致病菌菌株。= 84）。通过盘扩散法和PCR研究了这些分离株的电阻分布。此外，筛选菌株的禽类病因的毒力相关基因.COLI（APEC）。该研究表明，最高和最低的抗微生物性抗性分别对抗氧化胞菌素和庆大霉素。催产素，氟芬糖素和链霉素抵抗率致病性分离株高于非共生（P <0.05）。此外，TETA，BLATEM，ANDAAC（3）-iVResisce基因在致病性中比非共生分离株更普遍（P <0.05）。超过一半的分离物没有毒力相关的基因。多重PCR结果表明，在致病性比Commensale.coli（P <0.05）中致致病成分更为普遍。然而，其他基因并非如此。我们的结果表明抗菌性抗性抗性和毒力基因的低频率。鸵鸟的抗菌基因。抗菌剂易感性曲线和毒力基因含量。鸵鸟的科学酸盐酸盐不同于推定的共生和致病菌株。然而，需要更多的分析来区分这些隔离物。

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