• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Tropical Animal Health and Production >Identification and genetic analysis of infectious bursal disease viruses from field outbreaks in Kerala, India
【24h】

Identification and genetic analysis of infectious bursal disease viruses from field outbreaks in Kerala, India

机译：印度喀拉拉邦野外爆发的传染性育病毒鉴定及遗传分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Recurrent infectious bursal disease (IBD) outbreaks were reported in different regions of Kerala, India. This paper reports the comparative genetic analysis of the hypervariable region of the VP2 gene of IBD virus isolates from the field outbreaks in Kerala. In phylogenetic analysis, the obtained field isolates fall into genogroup 1 and 3. In genogroup 3, all vvIBDV isolates shared a common ancestor with other south Indian isolates but isolates 9/CVASP/IBDV, 10/CVASP/IBDV, 12/CVASP/IBDV, 14/CVASP/IBDV and 17/CVASP/IBDV are most recently evolved and are diverged from the south Indian isolates. The amino acid sequence of 22 isolates was analysed, out of which 18 had conserved amino acids which were characteristic of vvIBDV. All the vvIBDV isolates obtained in the study had phenylalanine and valine at the position 240 and 294, respectively, similar to recently evolved Indian IBDV isolate (MDI14). But we observed T269A and S299N mutations in the isolate 6/CVASP/IBDV, and it is the first report of such mutations at these positions in India IBDV isolates. The isolate 11/CVASP/IBDV had a unique mutation of V225A which is not yet reported in IBDV isolates. Two isolates (15/CVASP/IBDV and 18/CVASP/IBDV) were 100% amino acid similar to intermediate plus vaccine strain. The isolates 8/CVASP/IBDV/VP2 and 19/CVASP/IBDV had amino acids unique for the intermediate vaccine with mutations observed at H253Q and V256I in 19/CVASP/IBDV, T270A and novel mutation N279Y in isolate 8/CVASP/IBDV. These two isolates had non-virulent classical heptapeptide sequence 'SWSARGS'; nevertheless, they produce field outbreaks of IBD. This is the first report of genetic characterisation of IBDV in Kerala, India.
机译：在印度喀拉拉邦的不同地区报告了复发性传染性爆发（IBD）爆发。本文报道了IBD病毒分离株VP2基因的高变遗传分析与喀拉拉邦的田间爆发。在系统发育分析中，所获得的野外分离物落入基因组1和3.在基因组3中，所有VVIBDV分离物与其他南印度分离株共享共同的祖先，但分离9 / CVASP / IBDV，10 / CVASP / IBDV，12 / CVASP / IBDV最近最近演化的14 / CVASP / IBDV和17 / CVASP / IBDV，并从南印度分离株分叉分流。分析了22个分离物的氨基酸序列，其中18个具有VVIBDV的特征的保守氨基酸。在该研究中获得的所有VVIBDV分离物分别在240和294的位置具有苯丙氨酸和缬氨酸，类似于最近演化的印度IBDV分离物（MDI14）。但是，我们观察到分离物6 / CVASP / IBDV中的T269A和S299N突变，并且是印度IBDV分离株这些突变的第一个突变的第一报告。分离物11 / CVASP / IBDV具有v225a的独特突变，尚未在IBDV分离物中报道。两种分离株（15 / CVASP / IBDV和18 / CVASP / IBDV）为100％氨基酸，类似于中间体加疫苗菌株。分离株8 / CVASP / IBDV / VP2和19 / CVASP / IBDV具有在19 / CVASP / IBDV，T270A和新突变N279Y中在H253Q和V256I下观察到的中间疫苗具有突变的氨基酸，其中分离物8 / CVASP / IBDV。这两种分离物具有非毒性的经典七肽序列'Swsargs';尽管如此，它们会产生IBD的现场爆发。这是印度喀拉拉邦IBDV遗传表征的第一个报告。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号