• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Tropical Animal Health and Production >Single nucleotide polymorphisms at heat shock protein 90 gene and their association with thermo-tolerance potential in selected indigenous Nigerian cattle
【24h】

Single nucleotide polymorphisms at heat shock protein 90 gene and their association with thermo-tolerance potential in selected indigenous Nigerian cattle

机译：热休克蛋白90基因的单核苷酸多态性及其与选定的土着尼日利亚牛的热耐受潜力的关系

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Heat shock protein (HSP) 90 gene provides protection and adaptation to thermal assault and certain polymorphisms have been associated to heat tolerance in humans and animals. Single nucleotide polymorphisms (SNPs) of HSP 90 gene were used to evaluate the scientific basis of heat tolerance in four zebu breeds of Nigeria. The DNA was extracted from skin tissue of 90 adult bulls representing White Fulani (WF), Sokoto Gudali (SG), Red Bororo (RB), and Ambala (AM). The SNPs were determined in DNAs using PCR, sequencing, and visualization and bio-editing by chromatogram in SeqMan Ngen tool. Subsequently, respective genotypes were constructed and genotypic and allelic frequencies were computed. Also, body parameters related to heat stress (HS) including body temperature (BT), rectal temperature (RT), and respiratory rates (RR) were taken for each animal before biological sampling and heat tolerance coefficient (HTC) was calculated. We detected four SNPs distinct/specific for each breed as follows: change from thymine (T) to guanine (G) at position 116 (T116G) in RB, G to cytosine (C) at 220 (G220C) in SG, G to adenine (A) at two positions, 346 (G346A) and 390 (G390A) in AM and WF, respectively. Heterozygous SNPs showed significantly lower values (P < 0.0001) for BT, RT, RR, and HTC than homozygous genotypes at all positions. We hypothesize that animals with heterozygous SNPs in exon 3 of HSP 90 may be tolerant to HS. These SNPs can be used as bio-markers for screening large populations of cattle for tolerance to hot tropical conditions in Nigeria and other sub-humid places.
机译：热休克蛋白（HSP）90基因提供保护和适应热攻击，并且某些多态性与人类和动物的耐热耐受相关。 HSP 90基因的单一核苷酸多态性（SNP）用于评估尼日利亚四种Zebu品种的耐热性的科学基础。从代表白雀（WF），Sokoto Gudali（SG），Red Boro（RB）和Ambala（AM）的皮肤组织中提取DNA。在SEQMAN NGEN工具中，使用PCR，测序和可视化和生物编辑在DNA中测定SNP。随后，构建各种基因型，计算基因型和等位基因频率。此外，在计算生物采样和耐热系数（HTC）之前，对每只动物进行包含体温（BT），直肠温度（RT）和呼吸速率（RR）的热应激（HS）相关的体参数。我们检测到四个不同/特异性的SNP，如下：从SG，G至腺嘌呤的220（G220C）的RB，G至CytoSine（C）中的胸腺嘧啶（g）的胸腺嘧啶（g）的变化改变（a）分别在am和wf的两个位置，346（g346a）和390（g390a）。杂合子SNP对于BT，RT，RR和HTC显着较低的值（P <0.0001），而不是在所有位置的纯合基因型。我们假设HSP 90的外显子3中具有杂合子SNP的动物可以耐受HS。这些SNP可用作生物标记，用于筛选大量牛的牛，以耐受尼日利亚和其他潜水地点的热带条件。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号