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Determination of in vitro digestibility of forage species used in ruminant feeding

机译:反刍动物饲料中使用的饲料种类的体外消化率的测定

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Within the evaluation of the quality of forage resources, the main parameter that defines it is the digestibility of dry matter, which together with the amount of neutral and acidic detergent fibers and crude protein constitutes the basic information to assess forages which are supplied in the diet of the cattle. This research was carried out at the University of Los Llanos (Villavicencio, Colombia), and its objective was to determine the digestibility of three forages in cattle through three different in vitro techniques: inoculation with ruminal fluid and with feces and enzymatic digestibility technique, making the comparison with the in situ technique in order to validate the techniques and equipment that are being used for these procedures. The following species were evaluated:Pennisetum purpureum(PP),Tithonia diversifolia(TD), andBauhinia variegata(BV), assessing the curve and rate of degradation of dry matter (DM), neutral detergent fiber (NDF), and total protein (TP) (0 to 72 h). A design of repeated measures was used, under which the analysis of variance was carried out to determine the ranges of deviation between the techniques and thus establish the trend of the data; the variables evaluated were the DM, NDF, and TP digestibilities of the three forages using the four techniques (three in vitro and one in situ). After verifying the differences between the variances of the digestibilities and checking the sphericity assumption with the Mauchly test, multiple comparisons were made with the Bonferroni test with a significance of 5%. The digestibility of DM, NDF, and TP varied between 38.62 and 44.22, 54.18 and 66.97, and 47.54 and 57.05%; 49.07 and 70.70, 72.52 and 75.44, and 62.61 and 74.02%; 29.93 and 34.84, 26.21 and 70.88, and 25.67 and 50.60% respectively in forages PP, TD, and BV, depending on the technique used for their estimation. Despite finding statistically significant differences between several of the comparisons made in the digestibility techniques, a high coefficient of determination and a high correlation between the in vitro estimations with respect to the in situ estimation were found; therefore, it is possible to use these techniques routinely thus avoiding the need to have cattle with fistulae to perform digestibility tests, with enzymatic digestibility technique being the most practical one.
机译:在评估饲料资源质量的评估中,定义它的主要参数是干物质的消化率,其与中性和酸性洗涤剂纤维和粗蛋白质的数量构成了评估饮食中供应的饲料的基本信息牛。这项研究是在Los Llanos大学(Villavicencio,哥伦比亚)进行的,其目的是通过三种不同的体外技术确定牛中三个饲料的消化率:用瘤胃流体和粪便和酶消化技术接种,制作与原位技术的比较,以验证用于这些程序的技术和设备。评估以下物种:Pennisetum purpureum(pp),脂质植物血清(Td),andbauhinia variegata(bv),评估干物质(dm),中性洗涤剂纤维(ndf)和总蛋白质的降解曲线(Tp )(0至72小时)。使用重复措施的设计,在此进行方差分析以确定技术之间的偏差范围,从而确定数据的趋势;评估的变量是使用四种技术(三种体外和一个原位)的三种饲料的DM,NDF和TP消化。在验证消化力的差异和用摩毛型测试检查球形假设之间的差异之后,用硼管龙试验进行多种比较,其显着性为5%。 DM,NDF和TP的消化率在38.62和44.22,54.18和66.97之间变化,47.54和57.05%; 49.07和70.70,72.52和75.44,62.61和74.02%; Farage PP,TD和BV分别为29.93和34.84,26.21和70.88和25.67和50.60%,具体取决于用于其估计的技术。尽管在消化技术中的几个比较之间发现了统计上显着的差异,但发现了在消化技术中的几个比较之间的差异,发现了高度的确定系数和相对于原位估计的体外估计之间的高相关;因此,可以常规使用这些技术,从而避免需要牛与瘘管进行消化测试的需要,以酶消化技术是最实用的。

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