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首页> 外文期刊>Tropical Animal Health and Production >Virulence-associated genes and antimicrobial resistance among avian pathogenic Escherichia coli from colibacillosis affected broilers in Pakistan
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Virulence-associated genes and antimicrobial resistance among avian pathogenic Escherichia coli from colibacillosis affected broilers in Pakistan

机译：来自大肠杆菌的禽类致病大肠杆菌在巴基斯坦影响肉鸡的毒性相关基因和抗微生物抗性

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摘要

Avian pathogenic Escherichia coli (APEC) causes colibacillosis that leads to high morbidity and mortality among poultry birds. To date, there is a lack of knowledge about virulence-associated genes (VAGs) and multidrug resistance of APEC isolates from Pakistan. In this study, we determined the VAGs and antibiotic resistance profiles of APEC isolates recovered from colibacillosis affected broilers in Faisalabad region of Pakistan. A total of 84 diseased and dead birds from different local broilers farms were collected and examined for the gross lesions of colibacillosis by conducting postmortem examination. Of these, APEC isolates were recovered from 75 (89.2%) birds. Antibiotic susceptibility tests against 11 antimicrobial agents showed the highest resistance against ampicillin (98.6%) followed by tetracycline (97.3%) and ciprofloxacin (72%). The presence of 11 virulence associated genes (VAGs) was detected by multiplex polymerase chain reaction (PCR). Of the 75 APEC, 32 (42.6%) harbored > 5 VAGs. Most commonly found genes were increased serum survival (iss; 84%), iron transport (iutA; 74.6%), and colicin V (Co/V; 60%). Twenty-two isolates (29.3%) were found to possess a combination of VAGs; iss, tsh, iroN, and iutA, in addition to other VAGs. To the best of our knowledge, this is the first report on the detection of virulence-associated genes and multidrug resistance among APEC isolates in Pakistan. In the future, the strains with the predominant set of VAGs can be used for colibacillosis diagnosis and as a potential vaccine candidate.
机译：禽致病性大肠杆菌（APEC）导致大康菌病导致家禽鸟类中发病率高和死亡率。迄今为止，缺乏关于毒力相关基因（VAG）和来自巴基斯坦分离株的多药抗性的知识。在这项研究中，我们确定了在巴基斯坦Faisalabad地区的Colibacillosis受影响的肉鸡中回收的APEC分离物的Vags和抗生素抗性曲线。收集了来自不同当地肉鸡农场的84只患病和死亡鸟类，并通过进行后期检查来检查COLIBACILLIS的总病变。其中，从75（89.2％）鸟类中回收APEC分离物。对11种抗微生物剂的抗生素易感性试验表现出对氨苄青霉素（98.6％）的最高抗性，然后是四环素（97.3％）和环丙沙星（72％）。通过多重聚合酶链式反应（PCR）检测11个毒力相关基因（VAG）的存在。在75亚太组中，32名（42.6％）哈博尔博勒西州> 5 vag。最常见的基因增加了血清存活（ISS; 84％），铁运输（Iuta; 74.6％）和肠v（CO / V; 60％）。发现二十二分离株（29.3％）具有VAG的组合;除其他vags之外，ISS，TSH，Iron和Iuta还有。据我们所知，这是第一份关于检测巴基斯坦APEC隔离物中毒力相关基因和多药抗性的第一份报告。未来，具有主要型VAG的菌株可用于玉米菌诊断和潜在的疫苗候选者。

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