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首页> 外文期刊>Tropical Animal Health and Production >Co-inherited novel SNPs of theLIPEgene associated with increased carcass dressing and decreased fat-tail weight in Awassi breed
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Co-inherited novel SNPs of theLIPEgene associated with increased carcass dressing and decreased fat-tail weight in Awassi breed

机译：与胴体敷料增加和脂肪尾重量增加的联遗工与胴体敷料增加，脂肪尾重

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The lipase E hormone-sensitive (LIPE) enzyme is one of the lipolytic enzymes, and it plays a key role in the regulation of adipose tissue deposition. This study was conducted to investigate the possible association between theLIPEgene variations and the main body weight measurements in Awassi sheep. A total of 160 of sexually mature Awassi rams (Ovis aries) that aged between 2 and 3 years were included in the present study. Genomic DNA was extracted and two specific PCR amplicons were designed to amplify two coding regions within theLIPEgene. Genotyping experiments were performed using polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP). Two different SSCP banding patterns were identified, CC and CD in exon 2, and AA and AT in exon 9. Five novel single-nucleotide polymorphisms (SNPs) were detected by sequencing, namely g.151C > A and g.198C > T in exon 2, and g.213G > C, g.226G > T, and g.232A > C in exon 9. Haplotype block analysis showed strong linkage disequilibrium values between the two SNPs in exon 2 and the three SNPs in exon 9. Association analysis of haplotypes with carcass traits demonstrated a significantly higher dressing percentage (P < 0.05) and lower fat tail weight (FTW) in CACT and GCGTAC haplotypes made these haplotypes more favorable for human consumption. The current research is the first one to report a tight association between theLIPEgenetic polymorphism and the dressing percentage and FTW traits, suggesting a pivotal role played by these co-inherited SNPs in the metabolism of carcass traits in sheep.
机译：脂肪酶E激素敏感（LiPE）酶是脂肪溶解酶之一，并且它在脂肪组织沉积的调节中起着关键作用。进行该研究以研究北乙烯变异与AWASSI绵羊的主体重量测量之间的可能关联。在本研究中纳入2至3岁之间的160名性成熟的AWASSI RAM（OVIS白羊座）。提取基因组DNA，设计了两种特异性PCR扩增子以扩增三丙烯中的两个编码区。使用聚合酶链反应 - 单链构象多态性（PCR-SSCP）进行基因分型实验。在外显子2中鉴定出两种不同的SSCP条带模式，CC和Cd，AA和AT在外显子9.通过测序检测5个新的单核苷酸多态性（SNP），即G.151C> A和G.198C> T IN外显子2和G.226g> C，G.226g> T和G.232A> C在外显子9.单倍型嵌段分析显示出外显子2和外显子9中的三个SNP之间的两个SNP之间的强烈连锁不平衡值胴体性状的单倍型分析显示出明显较高的敷料百分比（P <0.05）和颈腺中的较低脂肪尾重（FTW），GCGTAC单倍型使得这些单倍型对人类消费更有利。目前的研究是第一个报告你的牙龈多态性和敷料百分比和FTW特征之间的紧张关联，这表明这些共遗传的SNP在绵羊中胴体性状的代谢中发挥了枢转作用。

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