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首页> 外文期刊>Tropical Animal Health and Production >Inhibition of NF-KB signaling pathway by astaxanthin supplementation for prevention of heat stress induced inflammatory changes and apoptosis in Karan Fries heifers
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Inhibition of NF-KB signaling pathway by astaxanthin supplementation for prevention of heat stress induced inflammatory changes and apoptosis in Karan Fries heifers

机译：抑制NF-KB信号传导途径通过虾青素预防热应激诱导炎症变化和Karan Friifers的细胞凋亡

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摘要

Present study was conducted on 12 Karan Fries (Holstein Friesian X Tharparkar) heifers (10-12 months) to assess the effect of astaxanthin supplementation on heat stress amelioration and inhibition of NF-KB signaling pathway for prevention of heat stress induced inflammatory changes and apoptosis in the cell during the summer season. The heifers were randomly and equally divided into two groups, i.e., control (fed as per ICAR 2013) and treatment groups (additionally supplemented astaxanthin at a dose rate of 0.25 mg/kg BW/day/animal). Temperature humidity index used to assess the levels of summer stress during the experimental period. Blood samples were collected at the fortnightly interval for quantification of plasma cortisol and IL-12 from both the groups of the heifers and from collected blood samples, RNA was isolated and transcribed into cDNA for real time PCR, for genes expression of NF-KB, IL-2, caspase-3, and Bcl-2. Plasma cortisol, IL-12 levels, and expression pattern of NF-KB, 1L-2, and caspase-3 were significantly (P 0.05) lower in treatment group of Karan Fries heifers than control group, whereas, Bcl-2 was higher (P 0.05) in astaxanthin supplemented group. The temperature humidity index had a positive correlation (P 0.05) with plasma cortisol and. IL-12 and expression pattern of NF-KB, IL-2, and caspase-3. However, it was negatively correlated with Bcl-2. The supplementation of astaxanthin can ameliorate the impact of summer stress through NF-KB downregulation, might be due to the quenching of free radicals, which regulates the expression of pro-inflammatory mediators and apoptotic genes.
机译：目前的研究是在12个Karan Fries（Holstein Friesian x Tharparkar）的小母牛（10-12个月）上进行，以评估虾青素补充对热应激改善和抑制NF-KB信号通路的抑制，以防止热应激诱导炎症变化和细胞凋亡在夏季的细胞中。继承者随机，同等地分为两组，即控制（根据ICAR 2013的喂养）和治疗组（以0.25mg / kg BW /天/动物的剂量率补充虾青蛋白）。温度湿度指数用于评估实验期间避暑胁迫水平。在每两周间隔内收集血液样品，用于定量血浆皮质醇和IL-12来自小母牛的组和来自收集的血液样品，分离RNA并转录成实时PCR的cDNA，用于NF-KB的基因表达， IL-2，Caspase-3和Bcl-2。血浆皮质醇，IL-12水平和NF-KB，1L-2和Caspase-3的表达模式显着（P <0.05）的治疗组，而不是对照组，而BCL-2是虾青素补充基团的较高（P <0.05）。温度湿度指数具有血浆皮质醇和血浆皮质醇的正相关（P <0.05）。 NF-KB，IL-2和Caspase-3的IL-12和表达模式。但是，它与BCL-2负相关。虾青素的补充可以改善夏季应力通过NF-KB下调的影响，可能是由于自由基的淬火，调节促炎介质和凋亡基因的表达。

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