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首页> 外文期刊>Tropical Animal Health and Production >Determination of antibiotic resistance pattern and virulence genes in Escherichia coli isolated from bovine with subclinical mastitis in southwest of Iran
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Determination of antibiotic resistance pattern and virulence genes in Escherichia coli isolated from bovine with subclinical mastitis in southwest of Iran

机译：伊朗西南西南北临床乳腺炎中脑内大肠杆菌中抗生素抗性模式和毒力基因的测定

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摘要

The aims of the present study were to investigate the prevalence of some virulence genes and also determine the antimicrobial resistance pattern of E. coli isolated from bovine with subclinical mastitis. The milk of 502 cows was collected from 8 dairy herds in the southwest of Iran. Conventional biochemical tests were used for identification of E. coli at the species level. Antimicrobial susceptibility patterns of E. coli isolates were determined by disc agar diffusion method and polymerase chain reaction (PCR) was used for detection of seven virulence genes including f17A, afaE-8, afaD-8, eaeA, cnf1, cnf2, and iucD. Seventy (13.94%) isolates of E. coli were identified in 502 milk samples. The highest rate of resistance was observed against tetracycline (18.6%), while none of the isolates were resistant to streptomycin. Eight (11.5%) out of 70 E. coli isolates carried at least one of the virulence genes. The afaD-8 was the most prevalent gene detected in 5 (7.1%) isolates. The afaE-8, iucD, and eaeA were detected in 3, 3, and 2 isolates respectively. Low prevalence of virulence factors may be indicating that most of the E. coli isolates originated from the commensal flora of cows and enter to the udders via environment contamination with feces.
机译：本研究的目的是研究一些毒力基因的患病率，并确定从亚临床乳腺炎中分离的大肠杆菌的抗微生物抗性模式。 502奶牛的牛奶从伊朗西南部的8个乳制品中收集。常规的生化试验用于鉴定物种水平的大肠杆菌。通过盘琼脂扩散法测定大肠杆菌分离株的抗微生物易感性模式和聚合酶链反应（PCR）用于检测七种毒力基因，包括F17A，AFAE-8，AFAD-8，EAEA，CNF1，CNF2和IUCD。七十（13.94％）在502种牛奶样品中鉴定了大肠杆菌的分离物。观察到四环素（18.6％）观察到最高的抗性率，而分离物没有抗链霉素。 70大肠杆菌分离物中的八个（11.5％）携带至少一种毒力基因。 AFAD-8是5（7.1％）分离株检测到最普遍的基因。在3,3和2分离株中检测到AFAE-8，IUCD和EAEA。毒力因子的低患病率可能表明大多数大肠杆菌分离物源于奶牛的非负面植物，并通过粪便的环境污染进入乌丁。

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