• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Tropical Animal Health and Production >First report on molecular characterization and phylogenetic analysis of Reticuloendotheliosis virus in Sudan
【24h】

First report on molecular characterization and phylogenetic analysis of Reticuloendotheliosis virus in Sudan

机译：苏丹网状枢轴病毒分子表征和系统发育分析的第一报告

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The reticuloendotheliosis virus (REV) group of retroviruses infects a wide range of avian species, including chickens, turkeys, ducks, geese, quail, and prairie chickens. The infection can result in immunosuppression, runting syndrome, high mortality, acute reticular cell neoplasia, or T- and/or B-cell lymphoma. One PCR positive chicken spleen sample obtained in a previous study in addition to one Marek & apos;s disease and three fowl pox (FP) vaccine samples were investigated in this study. A PCR assay was performed to detect the presence of REV provirus DNA in these samples. The results indicated the contamination of fowl pox virus and Marek & apos;s disease vaccines with REV. In addition, detection of integration of REV inside the genome of fowl pox vaccine was confirmed using primers corresponding to the FPV DNA regions flanking the REV integration site. Alignments of two sequences, one from the spleen tissue and the other from contaminated FP vaccine with REV, with other REV (env) gene sequences obtained from GenBank indicated their high similarity. Furthermore, phylogenetic analysis indicated that the partial part of (env) gene of our two isolates was closely related to variants from India, USA, Taiwan, and China. These results confirmed the contamination of commercial fowl pox and Marek's disease vaccines used in Sudan with REV. Phylogenetic analysis indicated that the partial part of (env) gene sequences from Sudan was closely related to variants from India, USA, Taiwan, and China.
机译：网状细胞病毒（Rev）逆转录病毒感染了广泛的禽类种类，包括鸡，火鸡，鸭子，鹅，鹌鹑和草原鸡。感染可导致免疫抑制，润滑综合征，高死亡率，急性网状细胞瘤或T-和/或B细胞淋巴瘤。在本研究中研究了在先前研究中，在先前的研究中获得的一种PCR正鸡脾样品，并在本研究中研究了三个禽毒液样品。进行PCR测定以检测这些样品中Rev Provirus DNA的存在。结果表明，具有Rev的Fowl Pox病毒和MAREK的疾病疫苗污染。此外，使用对应于REV集成位点的FPV DNA区域的引物来确认Rev的整合Rev内部的整合。两种序列的对准，其中一个来自脾组织，另一个来自污染的FP疫苗与Rev，与GeNBank获得的其他Rev（ENV）基因序列表示其高相似性。此外，系统发育分析表明，我们的两种分离株的（ENV）基因的部分部分与来自印度，美国，台湾和中国的变体密切相关。这些结果证实了苏丹使用的商业禽痘和MAREK病疫苗的污染。系统发育分析表明，苏丹（env）基因序列的部分部分与印度，美国，台湾和中国的变种密切相关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号