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首页> 外文期刊>Veterinary Immunology and Immunopathology >Incorporation of membrane-anchored flagellin into Salmonella Gallinarum bacterial ghosts induces early immune responses and protection against fowl typhoid in young layer chickens
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Incorporation of membrane-anchored flagellin into Salmonella Gallinarum bacterial ghosts induces early immune responses and protection against fowl typhoid in young layer chickens

机译：将膜固定鞭毛蛋白掺入沙门氏菌的Ballinarum细菌鬼魂诱导早期免疫反应和对年轻层鸡的禽伤寒保护

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The present study aimed to investigate whether the incorporation of flagellin, a TLR5 agonist, in the bacterial ghosts (BGs) of Salmonella Gallinarum can enhance protective immune responses against fowl typhoid, a septicemic disease of poultry, in chickens. BGs are empty cell envelopes derived from Gram-negative bacteria through the bacteriophage phiX174 gene E mediated lysis. In this study, the S. Gallinarum ghosts carrying flagellin were genetically constructed utilizing a lysis plasmid pJHL184-flagellin, designed for the coexpression of the flagellin and the lysis protein E. The adjuvant effect of flagellin was evaluated by immunizing seven day old brown nick layer chicks once orally with either S. Gallinarum-flagellin (SG-fliC) ghosts or S. Gallinarum (SG) ghosts alone. Our results showed that immunization with the SG-fliC ghosts elicited early and higher systemic (IgG) and mucosal (IgA) antibody responses compared to the SG ghosts alone, although not always statistically significant. Flow cytometric analysis of the CD3 + CD4 + and the CD3 + CD8 + T cell populations in peripheral blood mononuclear cells were higher in chickens immunized with the SG-fliC ghosts compared to the chickens vaccinated with the SG ghosts alone. Furthermore, the chickens immunized with SG-fliC ghosts exhibited significantly (p & 0.05) higher IL-6 and IFN-gamma responses compared to the chickens vaccinated with the SG ghosts alone. On challenge with the virulent S. Gallinarum wild type strain at 28th day post immunization, 5 of 10 birds died (50%) in case of SG-fliC ghost group while 60% (6 of 10 birds died) mortality was observed in the SG ghost group. Collectively, these results suggest that the expression of flagellin in SG ghosts improves antigen-specific humoral and cell mediated immune responses, and can enhance protective efficacy of the BG-based vaccines against the virulent challenges.
机译：本研究旨在调查鞭毛蛋白，TLR5激动剂，在沙门氏菌的细菌鬼（BGS）中的掺入是否能够增强鸡的禽伤寒的保护性免疫反应。 BGS是通过噬菌体PHIX174基因E介导的裂解衍生自革兰氏阴性细菌的空细胞包膜。在本研究中，携带鞭毛蛋白的S.Ballinarum鬼魂使用裂解质粒PJHL184-Flagellin遗传构建，所述裂解质粒PJHL184-Flagellin设计用于鞭毛蛋白和裂解蛋白E的共表达。通过免疫七天棕色缺口层来评价鞭毛蛋白的佐剂效果单独用S. Gallinarum-Flagellin（SG-Flic）鬼魂或S.Gallinarum（SG）鬼魂口腔口腔。我们的研究结果表明，与单独的SG幽灵相比，与SG-FLIC鬼的免疫引发了早期和更高的全身（IgG）和粘膜（IgA）抗体反应，尽管并不总是有统计学意义。与单独用SG幽灵接种的鸡相比，CD3 + CD4 +和外周血单个核细胞中的CD3 + CD8 + T细胞中的CD3 + CD8 + T细胞群中的CD3 + CD8 + T细胞群体较高。此外，与SG-叶片免疫的鸡呈现出显着（P＆amp; 0.05）较高的IL-6和IFN-Gamma响应，与单独用SG幽灵接种疫苗的鸡相比。在免疫后第28天的毒性S. Ballinarum野生型菌株的挑战中，在SG-Flic Ghost群的情况下死亡（50％）中的5个（50％），而在SG中观察到60％（10只禽类中死亡）死亡率鬼群。总的来说，这些结果表明，SG幽灵中的鞭毛蛋白的表达改善了抗原特异性的体液和细胞介导的免疫反应，并可以提高BG基疫苗对毒力挑战的保护效果。

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