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首页> 外文期刊>Veterinary Parasitology >Direct detection of Tritrichomonas foetus in cattle genital fluid trough loop mediated isothermal amplification of elongation factor 1 alpha 1
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Direct detection of Tritrichomonas foetus in cattle genital fluid trough loop mediated isothermal amplification of elongation factor 1 alpha 1

机译：直接检测牛生殖器流体槽环介导的伸长因子1α1的热扩增等温扩增

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摘要

Testing for Tritrichomonas foetus and exclusion of infected animals is an effective way of improving the reproductive efficiency in a herd. Conventional PCR is inherently more specific than the culture method and quantitative PCR can significantly increase the detection limit. Loop Mediated Isothermal DNA Amplification (LAMP) is gaining interest because the method does not require expensive equipment, specificity and sensitivity can be as high as quantitative PCR. The object of this study was to develop a sensitive and friendly test for point of-care detection of T. foetus. The LAMP test that targeted T. foams elongation factor 1 alpha 1 sequences showed high specificity. Sensitivity was 100-1000 times higher than that reached through culture, polymerase chain reaction or with a previously developed LAMP for 5.8 ribosomal sequences. Moreover, T. foetus detection could be performed without DNA purification from infected cervical vaginal mucus (CVM) or smegma samples. The tf-eflal LAMP method was tested for field detection with paper strips soaked in CVM from infected cows and the results were observed 90 min later. Direct detection of T. foetus in CVM with the tf-eflal LAMP showed high sensitivity and specificity, and an overall diagnostic odds ratio of 56 (CI: 13.3-235.0). The tf-eflal LAMP showed great potential for diagnosis and control of T. foams in resource-challenged regions.
机译：TritriChomonas胎儿和排除感染动物的测试是一种提高群体中生殖效率的有效方式。常规PCR固有地比培养方法更具体，并且定量PCR可以显着增加检测极限。环介导的等温DNA扩增（灯）是兴趣的，因为该方法不需要昂贵的设备，特异性和敏感性可以高达定量PCR。本研究的目的是为T.胎儿的护理点检测进行敏感和友好的测试。靶向T.泡沫伸长因子1α1序列的灯测试显示出高特异性。灵敏度比通过培养物，聚合酶链反应或以前显影的灯达到5-1000倍以上100-1000倍。此外，可以在没有来自受感染的宫颈阴道粘液（CVM）或侦探样品的情况下进行T.胎儿检测。测试TF-EFLAL灯法进行现场检测，用纸条从感染的奶牛浸泡在CVM中，并在后续90分钟观察结果。通过TF-EFLAL灯的CVM直接检测T.胎儿的诱导性和特异性高，整体诊断量为56（CI：13.3-235.0）。 TF-EFLAL灯显示出诊断和控制在资源挑战区域中的诊断和控制潜力。

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