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首页> 外文期刊>Veterinary Parasitology >Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex
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Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex

机译：用于检测几种echinococcus sensu sensu Lato复合物的糖果灯测定的开发

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摘要

Cystic echinococcosis represents a significant problem in human and animal health and constitutes one of the most severe Neglected Tropical Diseases prioritized by the World Health Organization. The etiological agent is the complex Echinococcus granulosus sensu lato (s. l.), composed of several species/genotypes. Diagnosis in the definitive host and molecular epidemiology studies are important points for cystic echinococcosis control. Here we developed a new copro-LAMP assay, LAMP EGSL, for diagnosis in the definitive host for simultaneous detection of Echinococcus granulosus sensu stricto (s. s.), Echinococcus ortleppi, and Echinococcus canadensis species. Also, the analytical sensitivity, specificity and plausibility of performance in a rural context of a previously reported species-specific LAMP reaction, was evaluated. Both reactions showed high analytical sensitivity values (10 fg-100 fg DNA) and did not show cross reaction with DNA from host or other helminthic parasites. LAMP EGSL was performed with samples from an endemic area. In addition, the alkaline hydrolysis of one E. granulosus s. s. adult parasite followed by specific LAMP to E. granulosus s. s. was performed in a laboratory with low resources from another cystic echinococcosis endemic area. The results obtained suggest that LAMP EGSL represents a potential tool for canine diagnosis that could be useful for cystic echinococcosis control programs. In addition, we showed that LAMP reaction for E. granulous s. s., E. ortleppi and E. canadensis specific detection, could be useful for molecular epidemiology studies applicable to the definitive host. Both reactions were performed in endemic, rural areas without sophisticated equipment.
机译：囊性超声波功能亢进是人类和动物健康的重大问题，构成世界卫生组织优先考虑的最严重忽视的热带疾病之一。病因是复杂的echinococcus smarulosus sensu Lato（s.1.），由几种物种/基因型组成。确定性宿主和分子流行病学研究中的诊断是囊性超声病变控制的重要点。在这里，我们开发了一种新的综合灯测定，灯EGSL，用于同时检测Echinococcus sensu Sticalo（s。），echinococcus ortleppp ppi和echinococcus canadensis物种的确定宿主中的诊断。此外，评估了在先前报道的特异性灯反应的农村背景下性能的分析敏感性，特异性和合理性。两种反应显示出高分析敏感值（10fg-100 fg DNA），并且没有显示来自宿主或其他蠕虫寄生虫的DNA的交叉反应。灯EGSL与来自地方区域的样品进行。此外，一种E.颗粒状水解的碱性水解。 s。成人寄生虫，然后是特定灯到E.颗粒组。 s。在实验室中进行，从其他囊性超声波病变区域的低资源进行。所获得的结果表明，灯EGSL代表犬诊断的潜在工具，其可用于囊性超声能量控制程序。此外，我们展示了E.颗粒式S的灯反应。 S.，E. OrtlePPI和E. Canadensis特异性检测，可用于适用于最终宿主的分子流行病学研究。两种反应都是在没有复杂设备的地方，农村地区进行。

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