...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Veterinary Parasitology >Cell mediated and innate immune responses in pigs following vaccination and challenge with Toxoplasma parasites
【24h】

Cell mediated and innate immune responses in pigs following vaccination and challenge with Toxoplasma parasites

机译：在疫苗接种后猪的细胞介导和先天免疫应答，并用弓形虫寄生虫攻击

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Toxoplasma gondii has a worldwide distribution and can infect almost all warm blooded animals including pigs and humans. This study aims to examine the immune responses induced in pigs following vaccination (live S48 tachyzoites) and/or challenge with T. gondii oocysts, through the examination of changes in levels of transcription in CD4, CD8 alpha, IFN-gamma, IL-12p35, CXCR3, MyD88. The experiment involved four groups of animals; pigs in group 1 (Challenged) (Chal) were challenged orally with (1 x 10(3) oocysts) on day 28 of the experiment. Pigs in group 2 (Vaccinated /Challenged) (Vac/Chal) were vaccinated (S48 isolate tachyzoites) on day 0, then challenged on day 28. The group 3 (Vaccinated) (Vac) animals were vaccinated (S48 isolate tachyzoites) on day 0 of the experiment. Finally the group 4 (control) pigs remained non-vaccinated and non-challenged. All animals were culled 6 weeks post challenge. At post mortem samples of retropharyngeal lymph node (RLN), mesenteric LN (MLN) and spleen were collected, RNA was extracted and cDNA synthesised. The results showed significant increases in IFN-gamma expression in samples from groups 1 (Chal) and 2 (Vac/Chal) (RLN) and groups 1, 2 and 3 (Vac) (spleen) and in MyD88 expression (RLN) in samples from groups 1, 2 and 3 compared to the group 4 (control) animals. Significant increases were also observed in CD8 alpha expression in group 1 (Chal) (RLN) and groups 1 and 2 (Vac/Chal) (RLN and MLN) compared against group 4 (control) and group 3 (Vac) respectively. Conversely, significant down regulation of CD4 and/or IL-12p35 transcription was found in at least one sample from groups 1 (Chal), 2 (Vac/Chal) and 3 (Vac) compared to group 4 (control) pigs. This study demonstrates that cell mediated and innate immune responses are generated in pigs following exposure to T. gondii parasites (oocysts or tachyzoites), key amongst them appear to be IFN-gamma, MyD88 and CD8 alpha.
机译：Toxoplasma Gondii具有全球分布，可以感染几乎所有温血动物，包括猪和人类。本研究旨在通过检查CD4，CD8α，IFN-Gamma，IL-12P35的转录水平变化，检查猪疫苗接种后（Live S48 Tachyzoites）和/或挑战攻击猪的免疫应答。 ，cxcr3，myd88。实验涉及四组动物;第1组（挑战）（Chal）的猪在实验的第28天口服（1 x 10（3）个卵囊）攻击。第2组（接种疫苗/攻击）（VAC / CHAL）的猪在第0天接种（S48分离出Tachyzoites），然后在第28天攻击。第3族（疫苗）（VAC）动物在一天中接种疫苗（S48分离出Tachyzoites） 0的实验。最后，第4组（对照）猪仍然是非接种疫苗和非挑战性的。所有动物都遭到挑战后6周。在逆床淋巴结（RLN）的后验鼠样本中，收集肠系膜LN（MLN）和脾，提取RNA并合成cDNA。结果表明IFN-Gamma表达在来自组1（Chal）和2（Vac / Chal）（RLN）和基团1,2和3（脾）和MyD88表达（RLN）中的样品中的IFN-Gamma表达的显着增加与第1组和3组相比，与第4组（对照）动物相比。在第1族（Chal）（RLN）和第1组（RLN）和第1组（RLN）和第2组（RLN和CHAL）（RLN和MLN）分别分别观察到在CD8α表达中观察到显着增加，与第4组（对照）和第3组（VAC）相比。相反，与第4组（对照）猪相比，在来自组1（Chal），2（Vac / Chal）和3（Vac）的至少一种样品中发现CD4和/或IL-12P35转录的显着调节。本研究表明，在暴露于Gondii寄生虫（卵囊或Tachyzoites）之后的猪中产生细胞介导和先天免疫应答，它们中的关键似乎是IFN-Gamma，MyD88和CD8α。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号