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首页> 外文期刊>Veterinary Parasitology >In vitro acaricidal activity of essential oil and alcoholic extract of Trachyspermum ammi against Dermanyssus gallinae
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In vitro acaricidal activity of essential oil and alcoholic extract of Trachyspermum ammi against Dermanyssus gallinae

机译:颅糊糊剂Ammi精油和酒精提取物的体外杀螨活性对抗Dermanyssus Gallinae

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摘要

This study aimed to assess in vitro acaricidal activity of essential oil (EO) and alcoholic extracts (AE) of Ajowan against D. gallinae. Using contact and spraying bioassays, different doses of EO and AE were tested. Cypermethrin and thymol (positive controls), and ethanol and distilled water (negative controls) were also tested. The results indicated that effects were method and dose dependent. Statistical analysis showed a highly significant difference for contact and spraying bioassays and various doses of EO and AE (p < 0.0001) as, the spraying method was more effective than the contact method for acaricidal activity at 24 h post treatment (PT). In this method, Cypermethrin, EO and thymol caused over 90 % mite mortality at 50 mu gcm(-2), while AE caused this rate at 150 mu gcm(-2). In the contact bioassay, all tests produced low mortality rates except for Cypermethrin. Thymol was the main constituent of EO (Area = 42.26 %) and AE (Area = 45.8 %). Results of the present study indicated that Ajowan had a satisfactory acaricidal effect against D. gallinae in vitro. It was also found that the spraying method could be used to control the D. gallinae as the most appropriate method.
机译:本研究旨在评估AJOWAN的精油(EO)和酒精萃取物(AE)对抗D. Gallinae的体外Acaricidal活性。使用接触和喷涂生物测定,测试了不同剂量的EO和AE。还测试了氯氰菊酯和晶醇(阳性对照)和乙醇和蒸馏水(阴性对照)。结果表明,效果是依赖于方法和剂量。统计学分析表明,接触和喷涂生物测定和各种剂量的EO和AE(P <0.0001)显示出高度显着差异,如图24h后24小时(PT)在24小时的杀螨活性接触方法更有效。在该方法中,Cypermethrin,EO和静脉醇在50μgccm(-2)下引起超过90%的螨虫死亡率,而Ae在150μmgcm(-2)下引起该速率。在接触生物测定中,除了硫酰菊酯外,所有测试都产生了低死亡率。胸腺醇是EO(面积= 42.26%)和Ae(面积= 45.8%)的主要成分。本研究结果表明,AJowan在体外对D. Gallinae进行了令人满意的敏锐效果。还发现喷涂方法可用于控制D. Gallinae作为最合适的方法。

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