Polymerase chain reaction - restriction fragment length polymorphism analysis for the differentiation of Trichinella nativa and Trichinella britovi

Mayer-Scholl A; Broglia A; Reckinger S; Nockler K

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Polymerase chain reaction - restriction fragment length polymorphism analysis for the differentiation of Trichinella nativa and Trichinella britovi

机译：聚合酶链反应 - 特希己素Nativa和Trichinella Britovi分化的限制性片段长度多态性分析

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Recently, Trichinella nativa was identified in foxes in Germany and Poland, indicating that the geographical distribution of T. nativa is not restricted to areas north of the isotherm -4degreeC in January. In the European Union, legislation requires that a regular monitoring of the occurrence of Trichinella spp. in indicator animals such as foxes or raccoon dogs be carried out. The Trichinella isolates must also be identified on a species level. The multiplex PCR recommended by the Community Reference Laboratory for Trichinella allows species identification, yet the differentiation of T. nativa and Trichinella britovi, a widespread Trichinella species in the temperate regions of Europe, is unstable. We therefore describe an easy and reliable method for the differentiation of the two species, which can be utilised to monitor a potential spread of T. nativa in Central Europe

机译：最近，Trichinella Nativa在德国和波兰的狐狸中鉴定，表明T.nativa的地理分布不仅限于1月份Isotherm -4degreec北部的地区。在欧盟，立法要求定期监测Trichinella SPP的发生。在指示剂如狐狸或浣熊等动物中进行。还必须在物种水平上鉴定Trichinella分离物。群体参考实验室为Trichinella推荐的多路复用PCR允许物种鉴定，然而，T.nativa和Trichinella Britovi的分化，欧洲温带的温带中普遍的Trichinella物种是不稳定的。因此，我们描述了一种简单可靠的方法，用于分化两种物种，可用于监测中欧的T. Nativa的潜在扩散

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来源
《Veterinary Parasitology》 |2014年第2期|共3页
作者
Mayer-Scholl A; Broglia A; Reckinger S; Nockler K;
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作者单位

Federal Institute for Risk Assessment Diedersdorfer Weg 1 12277 Berlin Germany;

European Food Safety Authority via Carlo Magno 1 43126 Parma Italy;

Federal Institute for Risk Assessment Diedersdorfer Weg 1 12277 Berlin Germany;

Federal Institute for Risk Assessment Diedersdorfer Weg 1 12277 Berlin Germany;

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原文格式 PDF
正文语种 eng
中图分类动物医学（兽医学）;
关键词
Trichinella; Foxes; Europe; RFLP; Restriction fragment length polymorphism; Trichinella nativa;

机译：Trichinella;狐狸;欧洲;RFLP;限制性片段长度多态性;Trichinella Nativa;

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Polymerase chain reaction - restriction fragment length polymorphism analysis for the differentiation of Trichinella nativa and Trichinella britovi

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