• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Processing of Infectious Bursal Disease Virus (IBDV)Polyprotein and Self-Assembly of IBDV-Like Particles in Hi-5 Cells
【24h】

Processing of Infectious Bursal Disease Virus (IBDV)Polyprotein and Self-Assembly of IBDV-Like Particles in Hi-5 Cells

机译：传染性法氏囊病病毒（IBDV）多蛋白的加工以及IBDV样颗粒在Hi-5细胞中的自组装

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The capsid of infectious bursal disease virus (IBDV),with a size of 60-65 nm,is formed by an initial processing of polyprotein (pVP2-VP4-VP3)by VP4,subsequent assemblage of pVP2 and VP3,and the maturation of VP2.In Sf9 cells,the processing of polyprotein expressed was restrained in the stage of VP2 maturation,leading to a limited production of capsid,i.e.,IBDV-like particles (VLPs).In the present study,another insect cell line,High-Five (Hi-5)cells,was demonstrated to efficiently produce VLPs.Meanwhile,in this system,polyprotein was processed to pVP2 and VP3 protein and pVP2 was further processed to the matured form of VP2.Consequently,Hi-5 cells are better in terms of polyprotein processing and formation of VLPs than Sf9.In addition to the processing of pVP2,VP3 was also degraded.With insufficient intact VP3 protein present for the formation of VLPs,the excessive VP2 form subviral particles (SVPs)with a size of about 25 nm.The ratio of VLPs to SVPs is dependent on the multiplicity of infections (MOIs)used,and an optimal MOI is found for the production of both particles.VLPs were separated from SVPs with a combination of ultracentrifugation and gel-filtration chromatography,and a large number of purified particles of both were obtained.In conclusion,the insect cell lines and MOIs were optimized for the production of VLPs,and pure VLPs with morphology similar to that of the wild-type viruses can be effectively prepared.The efficient production and purification of VLPs benefits not only the development of an antiviral vaccine against IBDV but also the understanding of the structure of this avian virus that is economically important.
机译：传染性法氏囊病病毒衣壳大小为60-65 nm，是由VP4对多蛋白（pVP2-VP4-VP3）进行初加工，随后pVP2和VP3组装以及VP2成熟而形成的在Sf9细胞中，表达多蛋白的过程在VP2成熟阶段受到抑制，导致衣壳即IBDV样颗粒（VLPs）的产生受到限制。在本研究中，另一种昆虫细胞系High-Five （Hi-5）细胞被证明可以有效地产生VLPs。同时，在该系统中，多蛋白被加工成pVP2和VP3蛋白，而pVP2被进一步加工成VP2的成熟形式。 VP3除了被pVP2加工外，还被降解。在没有完整的VP3蛋白质形成VLP的情况下，过量的VP2形成亚病毒颗粒（SVP），其大小约为25 VLP与SVP的比率取决于通过超速离心和凝胶过滤色谱法从SVPs中分离VLP，并获得了大量的纯化的颗粒。优化了昆虫细胞系和MOI以生产VLP，并可以有效制备形态类似于野生型病毒的纯VLP。高效生产和纯化VLP不仅有益于开发抗VLP的抗病毒疫苗IBDV以及对这种禽病毒结构的理解在经济上也很重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号