• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Evaluation of the Photosynthetic Reaction Center Protein for Potential Use as a Bioelectronic Circuit Element
【24h】

Evaluation of the Photosynthetic Reaction Center Protein for Potential Use as a Bioelectronic Circuit Element

机译：光合反应中心蛋白潜在用作生物电子电路元件的评估

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The characterization of a bioelectronic composite prepared by molecular wiring of a bacterial photosynthetic reaction center (RC) to a metal (Au) electrode is described.Two unique attachment sites on the protein surface were studied as sites for electrical connections-a polyhistidine tag introduced by site-directed mutagenesis and a native cysteine amino acid residue.These two attachment sites were evaluated independently and found to serve effectively in coupling the protein to the electrode surface asymmetrically.Cyclic voltammetry (CV) was used to monitor protein integrity and confirm protein chemisorption and orientation to the organofunctionalized gold electrode.Single-protein transport measurements made with conductive atomic force microscopy (C-AFM) were used to study the electrical transport.Current-voltage (I- V) curves obtained by wiring the protein at the polyhistidine tag showed diodelike behavior.The cysteine attachment site does not serve as an efficient means to address the protein electrically.Scanning tunneling spectroscopy (STS) performed on RCs coupled at the donor side under both dark-and white-light-illuminated conditions confirmed the C-AFM studies.
机译：描述了通过细菌光合作用反应中心（RC）与金属（Au）电极的分子布线制备的生物电子复合材料的特性。研究了蛋白质表面上的两个唯一附着位点作为电连接位点-由多聚组氨酸标签引入定点诱变和一个天然的半胱氨酸氨基酸残基，这两个连接位点被独立评估，发现可有效地将蛋白质不对称地偶联到电极表面上。循环伏安法（CV）用于监测蛋白质完整性并确认蛋白质的化学吸附和通过导电原子力显微镜（C-AFM）进行的单蛋白迁移测量来研究电迁移。通过在多组氨酸标签上连接蛋白获得的电流-电压（IV）曲线显示二极管样行为。半胱氨酸附着位点不能作为解决的有效手段在暗光和白光条件下，在供体侧耦合的RC上进行的扫描隧道光谱（STS）证实了C-AFM研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号