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首页> 外文期刊>Veterinary Research Communications >Electrophoretic detection of myeloperoxidase, protease, lactoferrin and lysozyme in buffalo polymorphonuclear granular acid extracts.
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Electrophoretic detection of myeloperoxidase, protease, lactoferrin and lysozyme in buffalo polymorphonuclear granular acid extracts.

机译：髓过氧化物酶，蛋白酶，乳铁蛋白和溶菌酶在水牛多核粒状酸提取物中的电泳检测。

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Polymorphonuclear (PMN) cells of more than 90% viability and 92% purity were isolated from the peripheral blood of four 3-year-old Murrah buffaloes. Following extraction of granular proteins from the cells, cationic proteins were extracted using 0.2mol/litre sodium acetate at pH 4.0 and were subjected to both non-denaturing and denaturing acid urea polyacrylamide gel electrophoresis (AUPAGE) to identify myeloperoxidase (MPO), lysozyme, protease activity and lactoferrin. Protease was identified using alpha-naphthyl acetate as substrate, while lactoferrin was identified using a reference lactoferrin from bovine milk in AUPAGE and by double immunodiffusion and Western blot techniques. Using AUPAGE, lysozyme was found to be the most cationic of all the proteins and peptides from the PMN granules. It is concluded that the granules in buffalo PMN cells have lysozyme, protease, MPO and lactoferrin.
机译：从四岁的Murrah水牛的四个3岁的Murrah Buffaloes的外周血中分离出超过90％的活力和92％纯度的多孔核（PMN）细胞。 从细胞中提取颗粒状蛋白质后，在pH 4.0下使用0.2mol /升乙酸钠萃取阳离子蛋白，并进行非变性和变性酸性尿素聚丙烯酰胺凝胶电泳（Aupage）以鉴定髓过氧化物酶（MPO），溶菌酶， 蛋白酶活性和乳铁蛋白。 使用α-萘氨酸酯作为底物鉴定蛋白酶，而使用来自Aupage的牛奶的参考乳铁蛋白和双免疫反转和Western印迹技术鉴定乳铁蛋白。 使用Aupage，发现溶菌酶是来自PMN颗粒的所有蛋白质和肽的最阳离子。 得出结论，水牛PMN细胞中的颗粒具有溶菌酶，蛋白酶，MPO和乳铁蛋白。

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