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首页> 外文期刊>Biotechnology Progress >Investigating the Kinetics of the Enzymatic Depolymerization of Polygalacturonic Acid in Continuous UF-Membrane Reactors
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Investigating the Kinetics of the Enzymatic Depolymerization of Polygalacturonic Acid in Continuous UF-Membrane Reactors

机译：在连续超滤膜反应器中研究聚半乳糖醛酸酶解聚的动力学

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摘要

A stirred tank membrane reactor is used to study the kinetics of polygalacturonic acid(PGA)enzymatic hydrolysis.The reactor operates in semicontinuous configuration:the native biopolymer is loaded at the initial time and the system is continuously fed with the buffer.The effect of retention time(from 101 to 142 min)and membrane molecular weight cutoff(from 1 to 30 kDa)on the rate of permeable oligomers production is investigated.Reaction products are clustered in two different classes,those sized below the membrane cutoff and those above,The reducing power measured in the permeate is used as an estimate of total product concentration.The characteristic breakdown times range from 40 to 100 min.The overall kinetics obeys a first-order law with a characteristic time estimated to 24 min.New mathematical data handling are developed and illustrated using the experimental data obtained.Finally,the body of the experimental results suggests useful indications(reactor productivity,breakdown induction period)for implementing the bioprocess at the industrial scale.
机译：搅拌釜式膜反应器用于研究聚半乳糖醛酸（PGA）酶水解的动力学。反应器以半连续构型运行：在初始时间加载天然生物聚合物，并向系统连续加入缓冲液。保留效果研究了渗透性低聚物产生速率的时间（101至142分钟）和膜分子量截留（1至30 kDa）。反应产物分为两类，其大小在膜截留以下和以上。在渗透液中测得的还原力被用作总产物浓度的估计值。特征分解时间为40至100分钟。总动力学服从一阶定律，特征时间估计为24分钟。新的数学数据处理方法是最后，实验结果的主体提供了有用的指示（反应器生产率，分解工业阶段），以实现工业规模的生物过程。

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