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Emergence of Ideal Membrane Cascades for Downstream Processing

机译:下游加工的理想膜级联的出现

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摘要

An algorithm is developed for describing ideal membrane cascades for fractionation of binary and pseudo-binary mixtures. It is shown that solvent management plays a key role in determining both purification and yield. Development of efficient diafilters is needed if membrane cascades are to achieve their full potential in competing with both chromatography and simulated moving bed operations in downstream processing of proteins. Such a replacement will also be important for fractionation of higher titers and larger substrates, such as plasmids, viruses, and even whole cells.
机译:开发了一种算法,用于描述用于分馏二元和伪二元混合物的理想膜级联反应。结果表明,溶剂管理在确定纯化和产率方面都起着关键作用。如果膜级联系统要在蛋白质的下游加工中与色谱法和模拟移动床操作相竞争,则需要开发高效的渗滤器。对于更高滴度和更大的底物(如质粒,病毒,甚至整个细胞)的分级分离,这种替代也很重要。

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