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首页> 外文期刊>Biotechnology Progress >Proteomic Profiling of Secreted Proteins from CHO Cells Using Surface-Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry
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Proteomic Profiling of Secreted Proteins from CHO Cells Using Surface-Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry

机译：使用表面增强激光解吸电离飞行时间质谱从CHO细胞分泌蛋白进行蛋白质组分析

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摘要

Chinese hamster ovary(CHO)cells are the most commonly used host cell line for the production of recombinant biopharmaceuticals.These biopharmaceuticals are typically secreted from CHO cells and purified from harvested cell culture media.The purpose of this study was to investigate changes in the secreted proteome of CHO cells over the various stages of the growth cycle using Surface Enhanced Laser desorption ionization time-of-flight mass spectrometry(SELDI-TOF MS).Conditioned media samples were collected each day over a 6 day growth period from CHO-K1 cells grown in low serum(0.5% FBS)conditions in monolayer culture.Samples were profiled on a number of ProteinChip arrays with different chromatographic surfaces.From this study,24 proteins were found to be differentially regulated at different phases of the growth cycle in CHO-K1 cells,when profiled on two chromatographic surfaces,Q10(anionic)and IMAC30(metal affinity)ProteinChip arrays.
机译：中国仓鼠卵巢（CHO）细胞是生产重组生物药物的最常用宿主细胞系，这些生物药物通常是从CHO细胞分泌的，并从收获的细胞培养基中纯化的，目的是研究分泌的细胞中的变化。使用表面增强激光解吸电离飞行时间质谱（SELDI-TOF MS），在生长周期各个阶段的CHO细胞蛋白质组学。在6天的生长期内，每天从CHO-K1细胞中收集条件培养基样品在低血清（0.5％FBS）条件下在单层培养中生长。在许多具有不同色谱表面的ProteinChip阵列上分析样品。从这项研究中，发现CHO-中24种蛋白质在生长周期的不同阶段受到不同的调控。 K1细胞，在Q10（阴离子）和IMAC30（金属亲和力）蛋白质芯片阵列的两个色谱图上进行分析。

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