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首页> 外文期刊>Biotechnology Progress >Establishment of Higher Passage PER.C6 Cells for Adenovirus Manufacture
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Establishment of Higher Passage PER.C6 Cells for Adenovirus Manufacture

机译：用于腺病毒生产的高传代PER.C6细胞的建立

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PER.C6 cells,an industrially relevant cell line for adenovirus manufacture,were extensively passaged in serum-free suspension cell culture to better adapt them to process conditions.The changes in ceUphysiology that occurred during this passaging were characterized by investigating cell growth,cell size,metabolism,and cultivation of replication-deficient adenovirus.The changes in cell physiology occurred gradually as the population doubling level,the number of times the cell population had doubled,increased.Higher passage PER.C6(HP PER.C6)proliferated at a specific growth rate of 0.043 h~(-1)2-fold faster than lower passage PER.C6,and were capable of proliferation from lower inoculation cell densities.HP PER.C6 cell volume was 16% greater,and cellular yields on glucose,lactate,oxygen,and amino acids were greater as well.In batch cultures,HP PER.C6 cells volumetrically produced 3-fold more adenovirus,confirmed with three different constructs.The increase in productivity was also seen on a cell-specific basis.Although HP PER.C6 were more sensitive to the"cell density effect",requiring lower infection cell densities for optimal specific productivity,they proliferated more after infection thanlower passage PER.C6,increasing the number of cells available for virus production.The extensive passaging established HP PER.C6 cells with several desirable attributes for adenovirus manufacture.
机译：PER.C6细胞是生产腺病毒的工业相关细胞系，已在无血清悬浮细胞培养物中广泛传代，以更好地适应过程条件。此传代过程中发生的细胞生理学变化的特征在于研究细胞生长，细胞大小复制缺陷型腺病毒的培养，代谢和培养。随着群体倍增水平，细胞数量翻倍的次数增加，细胞生理学的变化逐渐发生。更高的传代PER.C6（HP PER.C6）在比低传代PER.C6高0.043 h〜（-1）2倍的比生长速率，并且能够从较低的接种细胞密度进行增殖.HP PER.C6细胞体积增加16％，葡萄糖的细胞产量更高，在分批培养中，HP PER.C6细胞按体积产生的腺病毒数量增加了3倍，并证实了三种不同的构建体。尽管HP PER.C6对“细胞密度效应”更为敏感，但需要较低的感染细胞密度才能获得最佳的比生产力，但感染后它们的繁殖比低传代的PER.C6繁殖得更多，从而增加了可用于感染的细胞数量。广泛的传代建立了HP PER.C6细胞，具有腺病毒生产所需的几个属性。

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