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首页> 外文期刊>Biotechnology Progress >Design and Characterization of a Rotating Bed System Bioreactor for Tissue Engineering Applications
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Design and Characterization of a Rotating Bed System Bioreactor for Tissue Engineering Applications

机译：组织工程应用旋转床系统生物反应器的设计与表征

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摘要

The main challenge in the development of bioreactors for tissue engineering is the delivery of a sufficient nutrient and oxygen supply for cell growth in a 3D environment.Thus,a new rotating bed system bioreactor for tissue engineering applications was developed.The system consists of a culture vessel as well as an integrated rotating bed of special porous ceramic discs and a process control unit connected with the reactor to ensure optimal culturing conditions.The aim of the project was the design and construction of a fully equipped rotating bed reactor,and in particular,the characterization and optimization of the system with regard to technical parameters such as mixing time and pH-control to guarantee optimal conditions for cell growth and differentiation.Furthermore,the applicability of the developed system was demonstrated by cultivation of osteoblast precursor cells.The porous structure of the ceramic discs and the external medium circulation loop provide an optimal environment for tissue generation in long-term cultivations.Mass transfer limitations were minimized by the slow rotation,which also provides the cells with sufficient nutrients and oxygen through alternate contact to air and medium.An osteoblast precursor cell line was successfully cultivated in this bioreactor for 28 days.
机译：开发用于组织工程的生物反应器的主要挑战是为3D环境中的细胞生长提供足够的营养和氧气供应。因此，开发了一种用于组织工程应用的新型旋转床系统生物反应器。容器以及集成的特殊多孔陶瓷圆盘旋转床和与反应器连接的过程控制单元，以确保最佳的培养条件。该项目的目的是设计和建造设备齐全的旋转床反应器，特别是通过混合时间和pH控制等技术参数对系统进行表征和优化，以确保为细胞生长和分化提供最佳条件。此外，通过培养成骨细胞前体细胞证明了该系统的适用性。陶瓷盘片和外部介质循环回路提供了最佳环境缓慢旋转可最大程度地降低传质限制，并通过交替接触空气和培养基为细胞提供足够的营养和氧气。在该生物反应器中成功培养了28个成骨细胞前体细胞系天。

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