• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Multifrequency Permittivity Measurements Enable On-Line Monitoring of Changes in Intracellular Conductivity Due to Nutrient Limitations During Batch Cultivations of CHO Cells
【24h】

Multifrequency Permittivity Measurements Enable On-Line Monitoring of Changes in Intracellular Conductivity Due to Nutrient Limitations During Batch Cultivations of CHO Cells

机译：多频介电常数测量可以在线监测CHO细胞分批培养过程中由于营养限制引起的细胞内电导率变化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lab and pilot scale batch cultivations of a CHO K1/dhfr~- host cell line were conducted to evaluate on-line multifrequency permittivity measurements as a process monitoring tool. The β-dispersion parameters such as the characteristic frequency (f_C) and the permittivity increment (Δε_(max)) were calculated on-line from the permittivity spectra. The dual-frequency permittivity signal correlated well with the off-line measured biovolume and the viable cell density. A significant drop in permittivity was monitored at the transition from exponential growth to a phase with reduced growth rate. Although not reflected in off-line biovolume measurements, this decrease coincided with a drop in OUR and was probably caused by the depletion of glutamine and a metabolic shift occurring at the same time. Sudden changes in cell density, cell size, viability, capacitance per membrane area (C_M), and effects caused by medium conductivity (σ_m) could be excluded as reasons for the decrease in permittivity. After analysis of the process data, a drop in f_C as a result of a fall in intracellular conductivity (σ_i) was identified as responsible for the observed changes in the dual-frequency permittivity signal. It is hypothesized that the β-dispersion parameter f_C is indicative of changes in nutrient availability that have an impact on intracellular conductivity σ_i. On-line permittivity measurements consequently not only reflect the biovolume but also the physiological state of mammalian cell cultures. These findings should pave the way for a better understanding of the intracellular state of cells and render permittivity measurements an important tool in process development and control.
机译：进行了CHO K1 / dhfr_-宿主细胞系的实验室和中试规模的分批培养，以评估在线多频介电常数测量值，并将其作为过程监测工具。根据介电常数谱在线计算β色散参数，例如特征频率（f_C）和介电常数增量（Δε_（max））。双频介电常数信号与离线测量的生物量和活细胞密度密切相关。在从指数生长过渡到生长速率降低的阶段，介电常数显着下降。尽管未在离线生物量测量中反映出来，但这种下降与OUR下降同时发生，可能是由于谷氨酰胺的消耗和同时发生的代谢变化所引起的。作为介电常数降低的原因，可以排除细胞密度，细胞大小，活力，每膜面积电容（C_M）的突然变化以及中等电导率（σ_m）引起的影响。在分析过程数据之后，由于细胞内电导率（σ_i）下降而导致的f_C下降被认为是引起双频介电常数信号变化的原因。假设β-分散参数f_C指示营养物利用率的变化，该变化对细胞内电导率σ_i有影响。因此，在线介电常数测量不仅反映了生物体积，而且反映了哺乳动物细胞培养物的生理状态。这些发现将为更好地了解细胞的细胞内状态铺平道路，并使介电常数测量成为过程开发和控制中的重要工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号