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首页> 外文期刊>Biotechnology Progress >Growth Inhibition of Dinoflagellate Algae in Shake Flasks: Not Due to Shear This Time!
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Growth Inhibition of Dinoflagellate Algae in Shake Flasks: Not Due to Shear This Time!

机译：摇瓶中鞭藻藻类的生长抑制：这次不是由于剪切！

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摘要

Large scale algae cultures present interesting challenges in that they exhibit characteristics of typical bacterial and animal cell cultures. One current commercial food additive, docosahexaenoic acid (DHA), is produced using the dinoflagellate algae, Crypthecodinium cohnii. Like animal cell culture, the perceived sensitivity of algae culture to hydrodynamic forces has potentially limited the agitation and aeration applied to these systems. However, the high density cultivation of C cohnii required for an economically feasible process inevitably results in high oxygen demand. In this study, we demonstrated what first appeared to be a problem with shear sensitivity in shake flasks is most probably a mass transfer limitation. We subsequently demonstrated the limit of chronic and rapid energy dissipation rate, EDR, that C. cohnii cells can experience. This limit was determined using a microfluidic device connected in a recirculation loop to a stirred tank bioreactor, which has been previously used to repeatedly expose animal cells to high levels of EDR. Inhibition of cell growth was observed when C. cohnii cells were subjected to an EDR of 5.9 × 10~6 W/m~3 with an average frequency of 0.2/min or more. This level of EDR is sufficiently high that C. cohnii can withstand typically encountered hydrodynamic forces in bioprocesses. This result suggests that at least one dinoflagellate algae, C. cohnii , is' quite robust with respect to hydrodynamic forces and the scale-up of process using this type of algae should be more concerned with providing sufficient gas transfer given the relatively high oxygen demand.
机译：大型藻类培养物表现出有趣的挑战，因为它们表现出典型的细菌和动物细胞培养物的特征。一种当前的商业食品添加剂二十二碳六烯酸（DHA）是使用二鞭毛藻藻类Crypthecodinium cohnii生产的。像动物细胞培养一样，藻类培养物对流体动力的敏感度潜在地限制了应用于这些系统的搅拌和通气。然而，经济上可行的方法所需要的高密度培养隐孢子虫必然导致对氧气的高需求。在这项研究中，我们证明了摇瓶中最初出现的剪切敏感性问题最有可能是传质限制。随后，我们证明了C. cohnii细胞可以经历的慢性和快速能量耗散率EDR的极限。使用在循环回路中连接到搅拌槽生物反应器的微流体装置确定该极限，该装置先前已用于将动物细胞反复暴露于高水平的EDR。当梭状芽胞杆菌细胞受到EDR 5.9×10〜6 W / m〜3，平均频率为0.2 / min或更高时，观察到细胞生长受到抑制。 EDR的水平足够高，使得梭菌可以承受生物过程中通常遇到的流体动力。这一结果表明，至少一种鞭毛藻藻（C. cohnii）在流体动力方面非常稳健，鉴于氧气需求量相对较高，使用这种藻类的工艺规模扩大应更关注提供足够的气体转移。

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