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首页> 外文期刊>Biotechnology Progress >Impact of Antibody Aggregation on a Flowthrough Anion-exchange Membrane Process
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Impact of Antibody Aggregation on a Flowthrough Anion-exchange Membrane Process

机译：抗体聚集对流通式阴离子交换膜过程的影响

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摘要

The impact of typical anion-exchange flowthrough conditions on the IgG mass loading of an anion-exchange membrane scale-down unit (Mustang Q coin) was investigated. High performance size-exclusion chromatography and multiangle laser light scattering results suggested the presence of a small fraction of IgG aggregates with average radius >100 nm under anion-exchange flowthrough conditions. The small filtration area presented by the 0.35 mL membrane volume Mustang Q coin limited the membrane throughput due to fouling from the aggregates at higher antibody loading. Data in this report indicated that a 0.2 μm hybrid polyethersulfone and polyvinylidene fluoride membrane in-line preftlter with a minimum filtration area of 20 sq cm alleviated the Mustang Q coin fouling. The combined cake filtration and intermediate blocking model was proposed as the most likely membrane pore blocking mechanism. Increasing the filtration area in the in-line prefilter resulted in higher IgG mass throughput. Thus, using an appropriately sized in-line prefilter could provide more robust antibody throughput performance on scale-down membrane anion-exchange units.
机译：研究了典型的阴离子交换流通条件对阴离子交换膜按比例缩小装置（Mustang Q币）的IgG质量负载的影响。高性能尺寸排阻色谱和多角度激光散射结果表明，在阴离子交换流通条件下，平均半径> 100 nm的小部分IgG聚集体的存在。 0.35 mL膜体积的Mustang Q硬币呈现出的小过滤面积限制了膜通量，因为在较高的抗体负载下聚集体会结垢。该报告中的数据表明，最小过滤面积为20平方厘米的0.2μm混合聚醚砜和聚偏二氟乙烯膜在线预滤器减轻了Mustang Q硬币的结垢。滤饼过滤和中间阻塞的组合模型被认为是最可能的膜孔阻塞机制。在线预过滤器中过滤面积的增加导致更高的IgG质量通量。因此，使用适当大小的在线预滤器可以在按比例缩小的膜阴离子交换单元上提供更强大的抗体通量性能。

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