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首页> 外文期刊>Biotechnology Progress >Effect of Needle Geometry on Flow Rate and Cell Damage in the Dispensing-Based Biofabrication Process
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Effect of Needle Geometry on Flow Rate and Cell Damage in the Dispensing-Based Biofabrication Process

机译：基于点胶的生物制造过程中针头几何形状对流速和细胞损伤的影响

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摘要

Biodispensing techniques have been widely applied in biofabrication processes to deliver cell suspensions and biomaterials to create cell-seeded constructs. Under identical operating conditions, two types of dispensing needles-tapered and cylindrical-can result in different flow rates of material and different cell damage percent induced by the mechanical forces. In this work, mathematical models of both flow rate and cell damage percent in biodispensing systems using tapered and cylindrical needles, respectively, were developed, and experiments were carried out to verify the effectiveness of the developed models. Both simulations and experiments show tapered needles produce much higher flow rates under the same pressure conditions than cylindrical needles. Use of a lower pressure in a tapered needle can therefore achieve the same flow rate as that in a cylindrical needle. At equivalent flow rates, cell damage in a tapered needle is lower than that in a cylindrical one. Both Schwann cells and 3T3 fibroblasts, which have been widely used in tissue engineering, were used to validate the cell damage models. Application of the developed models to specify the influence of process parameters, including needle geometry and air pressure, on the flow rate and cell damage percent represents a significant advance for biofabrication processes. The models can be used to optimize process parameters to preserve cell viability and achieve the desired cell distribution in dispensing-based biofabrication.
机译：生物分配技术已广泛应用于生物制造过程中，以递送细胞悬浮液和生物材料以创建细胞接种的构建体。在相同的工作条件下，锥形和圆柱形两种分配针会导致不同的物料流速和机械力引起的不同的细胞破损率。在这项工作中，分别建立了使用锥形和圆柱形针头的生物分配系统中流速和细胞损伤百分率的数学模型，并进行了实验以验证所开发模型的有效性。模拟和实验均显示，在相同压力条件下，锥形针比圆柱形针产生更高的流速。因此，在锥形针中使用较低的压力可以获得与圆柱形针相同的流速。在相同的流速下，锥形针的细胞损伤低于圆柱形针。被广泛用于组织工程的Schwann细胞和3T3成纤维细胞均被用于验证细胞损伤模型。应用开发的模型指定工艺参数（包括针的几何形状和气压）对流速和细胞损伤百分比的影响代表了生物制造工艺的重大进步。该模型可用于优化工艺参数，以保持细胞活力并在基于点胶的生物加工中实现所需的细胞分布。

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