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首页> 外文期刊>Biotechnology Progress >Process Evaluations and Economic Analyses of Recombinant Human Lysozyme and Hen Egg-White Lysozyme Purifications
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Process Evaluations and Economic Analyses of Recombinant Human Lysozyme and Hen Egg-White Lysozyme Purifications

机译：重组人溶菌酶和鸡蛋清溶菌酶纯化的工艺评价和经济分析

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摘要

Human lysozyme and hen egg-white lysozyme have antibacterial, antiviral, and antifungal properties with numerous potential commercial applications. Currently, hen egg-white lysozyme dominates low cost applications but the recent high-level expression of human lysozyme in rice could provide an economical source of lysozyme. This work compares human lysozyme and hen egg-white lysozyme adsorption to the cation exchange resin, SP-SepharoseTM FF, and the effect of rice extract components on lysozyme purification. With one exception, the dynamic binding capacities of human lysozyme were lower than those of hen egg-white at pH 4.5, 6, and 7.5 with ionic strengths ranging from 0 to 100 mM (5-20 mS). Ionic strength and pH had a similar effect on the adsorption capacities, but human lysozyme was more sensitive to these two factors than hen egg-white lysozyme. In the presence of rice extract, the dynamic binding capacities of human and hen egg-white lysozymes were reduced by 20-30% and by 32-39% at pH 6. Hen egg-white lysozyme was used as a benchmark to compare the effectiveness of human lysozyme purification from transgenic rice extract. Process simulation and cost analyses for human lysozyme purification from rice and hen egg-white lysozyme purification from egg-white resulted in similar unit production costs at 1 ton per year scale.
机译：人溶菌酶和鸡蛋清溶菌酶具有抗菌，抗病毒和抗真菌特性，具有许多潜在的商业用途。当前，鸡蛋清溶菌酶在低成本应用中占主导地位，但是最近人类在水稻中溶菌酶的高水平表达可以提供一种经济的溶菌酶来源。这项研究比较了人溶菌酶和鸡蛋清溶菌酶对阳离子交换树脂SP-SepharoseTM FF的吸附以及大米提取物成分对溶菌酶纯化的影响。除了一个例外，人溶菌酶的动态结合能力低于蛋清在pH 4.5、6和7.5时的动态结合能力，离子强度范围为0至100 mM（5-20​​ mS）。离子强度和pH值对吸附能力的影响相似，但是人溶菌酶对这两个因素的影响比鸡蛋清溶菌酶更敏感。在存在大米提取物的情况下，在pH 6时，人和鸡卵清溶菌酶的动态结合能力降低了20％至30％，而鸡蛋清溶菌酶的动态结合能力则降低了32-39％。转基因水稻提取物中人溶菌酶的纯化用于从大米中纯化人溶菌酶和从蛋清中纯化母鸡蛋白的溶菌酶的过程模拟和成本分析导致了每年1吨规模的相似单位生产成本。

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