...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Hearing Research: An International Journal >Concurrent gradients of ribbon volume and AMPA-receptor patch volume in cochlear afferent synapses on gerbil inner hair cells
【24h】

Concurrent gradients of ribbon volume and AMPA-receptor patch volume in cochlear afferent synapses on gerbil inner hair cells

机译：耳蜗内毛细胞耳蜗传入突触中的带状体积和AMPA受体贴剂的并发梯度

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Mongolian gerbil is a classic animal model for age-related hearing loss. As a prerequisite for studying age-related changes, we characterized cochlear afferent synaptic morphology in young adult gerbils, using immunolabeling and quantitative analysis of confocal microscopic images. Cochlear wholemounts were triple-labeled with a hair-cell marker, a marker of presynaptic ribbons, and a marker of postsynaptic AMPA-type glutamate receptors. Seven cochlear positions covering an equivalent frequency range from 0.5 - 32 kHz were evaluated. The spatial positions of synapses were determined in a coordinate system with reference to their individual inner hair cell. Synapse numbers confirmed previous reports for gerbils (on average, 20-22 afferents per inner hair cell). The volumes of presynaptic ribbons and postsynaptic glutamate receptor patches were positively correlated: larger ribbons associated with larger receptor patches and smaller ribbons with smaller patches. Furthermore, the volumes of both presynaptic ribbons and postsynaptic receptor patches co-varied along the modiolar-pillar and the longitudinal axes of their hair cell. The gradients in ribbon volume are consistent with previous findings in cat, guinea pig, mouse and rat and further support a role in differentiating the physiological properties of type I afferents. However, the positive correlation between the volumes of pre- and postsynaptic elements in the gerbil is different to the opposing gradients found in the mouse, suggesting species-specific differences in the postsynaptic AMPA receptors that are unrelated to the fundamental classes of type I afferents. (C) 2018 Elsevier B.V. All rights reserved.
机译：蒙古·阿伯利是一个经典动物模型，可与年龄相关的听力损失。作为学习年龄相关变化的先决条件，我们在年轻成人Gerbils中表征了耳蜗传入突触形态，使用免疫标签和共聚焦微观图像的定量分析。耳蜗形式是用毛细胞标记，突触前带的标记和突触后AMPA型谷氨酸受体的标志物的三重标记。评估覆盖0.5-22 kHz等效频率范围的七个耳蜗位置。参考其各个内毛细胞在坐标系中确定突触的空间位置。 Synapse号码确认了Gerbils的先前报告（平均而言，每内部毛细胞20-22引起）。突触线带和后腹膜谷氨酸受体贴片的体积呈正相关：与较大的受体贴片和具有较小贴片的较小丝带相关的较大的核心。此外，突触线带和突触后受体贴片的体积沿着模胶柱和其毛孔的纵向轴相同。带状体积中的梯度与猫，豚鼠，小鼠和大鼠的先前研究结果一致，进一步支持区分I型交感器的生理特性的作用。然而，Gerbil中的前后元素的量与突触后元素之间的正相关性与小鼠中发现的相对梯度不同，表明与I传入的基本类别无关的突触后AMPA受体的特异性差异。 （c）2018 Elsevier B.v.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号