• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Carassius auratus-Originated Recombinant Histone H1 C-Terminal Peptide as Gene Delivery Material
【24h】

Carassius auratus-Originated Recombinant Histone H1 C-Terminal Peptide as Gene Delivery Material

机译：assi鱼起源的重组组蛋白H1 C-末端肽作为基因传递材料

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The effective delivery of exogenous genes into eukaryotic cells is important for fundamental and biotechnological research.Protein-based gene delivery including histone proteins has recently emerged as a powerful technique for non-viral DNA transfer.Histones are DNA-binding proteins that function in DNA packaging and protection.In particular,histone H1 is largely responsible for the stabilization of higher-order chromatin structures.Several studies have examined the use of full-length histone H1-mediated gene transfer,and a few studies have investigated the use of C-terminal histone H1 fragments as gene-transfer materials.Previously,we cloned a novel histone H1 cDNA from the goldfish Carassius auraius and found that a recombinant histone H1 C-terminal short peptide(H1C)of 61 amino acids has comparable DNA binding and protection functions as full-length histone H1.In the present work,we successfully expressed and purified soluble recombinant H1C in an Escherichia coli expression system using a hexahistidine tag fusion strategy and providing tRNAs for rare codons.We confirmed its DNA-binding ability and found that this H1C peptide had similar or higher transfection efficiency in mammalian cells(human 293T and mouse NIH/3T3)than the widely used agent lipofectamine.Therefore,we suggest that this novel goldfish-derived recombinant histone H1 C-terminal short peptide could be used as a peptide-based gene-transfer mediator.
机译：有效地将外源基因传递到真核细胞中对基础和生物技术研究非常重要。基于蛋白质的基因传递（包括组蛋白）最近已成为一种有效的非病毒DNA转移技术。组蛋白是在DNA包装中起作用的DNA结合蛋白。特别是，组蛋白H1在很大程度上负责高阶染色质结构的稳定化。数项研究研究了全长组蛋白H1介导的基因转移的使用，而一些研究研究了C末端的使用以前，我们从金鱼Car鱼（Carassius auraius）克隆了一个新的组蛋白H1 cDNA，发现具有61个氨基酸的重组组蛋白H1 C端短肽（H1C）具有与DNA相似的DNA结合和保护功能。全长组蛋白H1。在本工作中，我们成功地在大肠杆菌表达系统中表达并纯化了可溶性重组H1C。我们证实了它的DNA结合能力，发现这种H1C肽在哺乳动物细胞（人293T和小鼠NIH / 3T3）中的转染效率与广泛使用的lipofectamine相似或更高。因此，我们建议，这种新颖的金鱼来源的重组组蛋白H1 C末端短肽可以用作基于肽的基因转移介体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号