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首页> 外文期刊>Biotechnology Progress >Synergistic Effect of Immobilized Laminin and Nerve Growth Factor on PC12 Neurite Outgrowth
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Synergistic Effect of Immobilized Laminin and Nerve Growth Factor on PC12 Neurite Outgrowth

机译：固定化层粘连蛋白和神经生长因子对PC12神经突生长的协同作用

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Immobilized extracellular matrix proteins and neurotrophins have been extensively studied to enhance neuronal adhesion and proliferation on surfaces for applications in nerve tissue engineering and neuroprosthetic devices.This article describes how the coimmobilization of laminin,an extracellular matrix protein and nerve growth factor(NGF),a neurotrophin can enhance neurite outgrowth observed separately with each type of molecule.In the absence of immobilized NGF,PC12 neurite outgrowth is influenced strongly by the presence of NGF in solution and unaffected by significant increases in laminin surface density(18.7-93.5 ng/ mm~2 ).However,when both laminin and NGF are immobilized together,the surface density of laminin is an important factor in determining whether or not the neurite outgrowth-promoting effect of NGF can be obtained.PC12 neurite outgrowth on surfaces with coimmobi-lized laminin and NGF with surface densities of 27.6 ng/mm~2 and 1.4 ng/mm~2,respectively,are similar to that observed on surfaces with immobilized laminin and dissolved NGF.
机译：固定化的细胞外基质蛋白和神经营养蛋白已被广泛研究以增强神经元在表面的粘附和增殖，可用于神经组织工程和神经修复装置。本文介绍层粘连蛋白，细胞外基质蛋白和神经生长因子（NGF）的共固定化神经营养蛋白可以增强每种分子分别观察到的神经突生长。在没有固定化NGF的情况下，PC12神经突生长受到溶液中NGF的强烈影响，而不受层粘连蛋白表面密度（18.7-93.5 ng / mm〜 2）。但是，当层粘连蛋白和NGF固定在一起时，层粘连蛋白的表面密度是确定能否获得NGF促进神经突生长的重要因素。和NGF的表面密度分别为27.6 ng / mm〜2和1.4 ng / mm〜2在固定有层粘连蛋白和NGF溶解的表面上观察到。

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