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首页> 外文期刊>Biotechnology Progress >Inhibition of Succinic Acid Production in Metabolically Engineered Escherichia Coli by Neutralizing Agent,Organic Acids,and Osmolarity
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Inhibition of Succinic Acid Production in Metabolically Engineered Escherichia Coli by Neutralizing Agent,Organic Acids,and Osmolarity

机译：中和剂，有机酸和渗透压抑制代谢工程大肠杆菌中琥珀酸的产生

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The economical viability of biochemical succinic acid production is a result of many processing parameters including final succinic acid concentration,recovery of succinate,and the volumetric productivity.Maintaining volumetric productivities >2.5 g L~(-1)h~(-1)is important if production of succinic acid from renewable resources should be competitive.In this work,the effects of organic acids,osmolarity,and neutralizing agent(NH4OH,KOH,NaOH,K2CO3,and Na2CO3)on the fermentative succinic acid production by Escherichia coli AFP 184 were investigated.The highest concentration of succinic acid,77 g L~,was obtained with Na2CO3.In general,irrespective of the base used,succinic acid productivity per viable cell was significantly reduced as the concentration of the produced acid increased.Increased osmolarity resulting from base addition during succinate production only marginally affected the productivity per viable cell.Addition of the osmoprotectant glycine betaine to cultures resulted in an increased aerobic growth rate and anaerobic glucose consumption rate,but decreased succinic acid yield.When using NH4OH productivity completely ceased at a succinic acid concentration of ~40 g L~(-1).Volumetric productivities remained at 2.5 g L~(-1)h~(-1)for up to 10 h longer when K- or Na-bases where used instead of NH4OH.The decrease in cellular succinic acid productivity observed during the anaerobic phase was found to be due to increased organic acid concentrations rather than medium osmolarity.
机译：生化琥珀酸生产的经济可行性是许多工艺参数的结果，包括最终琥珀酸的浓度，琥珀酸的回收率和容积生产率。保持容积生产率> 2.5 g L〜（-1）h〜（-1）很重要这项工作中，有机酸，渗透压和中和剂（NH4OH，KOH，NaOH，K2CO3和Na2CO3）对大肠杆菌AFP 184生产发酵型琥珀酸的影响用Na2CO3可获得最高浓度的琥珀酸77 g L〜。通常，无论使用哪种碱，随着产生的酸浓度的增加，每个活细胞的琥珀酸生产率都显着降低。渗透压升高在琥珀酸盐生产过程中添加碱所产生的影响仅轻微影响了每个活细胞的生产力。向培养物中添加渗透保护剂甘氨酸甜菜碱会导致有氧生长速率和厌氧葡萄糖消耗速率增加，但琥珀酸产量降低。当使用NH4OH时，当琥珀酸浓度约为40 g L〜（-1）时生产力完全停止，体积生产率保持在2.5 g L〜（-1）当使用K-或Na-碱代替NH4OH时，h〜（-1）最多可延长10 h。在厌氧阶段观察到的细胞琥珀酸生产率的下降是由于增加了有机酸浓度而不是介质渗透压。

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