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首页> 外文期刊>Biotechnology Progress >Preparation and Recycling of Aqueous Two-Phase Systems with pH-Sensitive Amphiphilic Terpolymer PADB
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Preparation and Recycling of Aqueous Two-Phase Systems with pH-Sensitive Amphiphilic Terpolymer PADB

机译：pH敏感两性三元共聚物PADB的水两相系统的制备和回收

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In this study,a novel pH-sensitive terpolymer PADB was synthesized by random terpoly-merization of 2-(dimethylamino) ethyl methacrylate,acrylic acid,and butyl methacrylate. The terpolymer PADB could form aqueous two-phase systems (ATPS) with a light-sensitive terpolymer PNBC,which was synthesized in our laboratory,using n-isopropylacrylamide,n-butyl acrylate,chlorophyllin sodium copper salt as monomers. More than 97% of the PADB terpolymer could be recovered by adjusting the pH to isoelectric point (PI) 4.1. The terpolymer PNBC could be recovered by using light radiation at 488 nm,with recovery ratio of 98%. BSA and lysozyme were partitioned in the PNBC-PADB ATPS to examine this new system. It was found that the partition coefficient of BSA and lysozyme could reach 4.46 and 0.49 in the systems,respectively.
机译：本研究通过甲基丙烯酸2-（二甲氨基）乙酯，丙烯酸和甲基丙烯酸丁酯的无规三元聚合反应合成了一种新型的pH敏感三元共聚物PADB。三元共聚物PADB可以与光敏三元共聚物PNBC形成水两相体系（ATPS），该三元共聚物是在我们的实验室中以正异丙基丙烯酰胺，丙烯酸正丁酯，叶绿素钠铜盐为单体合成的。通过将pH调节至等电点（PI）4.1，可以回收超过97％的PADB三元共聚物。三元共聚物PNBC可通过488nm的光辐射回收，回收率为98％。在PNBC-PADB ATPS中分配了BSA和溶菌酶，以检查此新系统。结果表明，系统中BSA和溶菌酶的分配系数分别达到4.46和0.49。

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