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首页> 外文期刊>Biotechnology Progress >Design and Characterization of a Modified T-Flask Bioreactor for Continuous Monitoring of Engineered Tissue Stiffness
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Design and Characterization of a Modified T-Flask Bioreactor for Continuous Monitoring of Engineered Tissue Stiffness

机译：连续监测工程组织刚度的改良T瓶生物反应器的设计和表征

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摘要

Controlling environmental conditions, such as mechanical stimuli, is critical for directing cells into functional tissue. This study reports on the development of a bioreactor capable of controlling the mechanical environment and continuously measuring force-displacement in engineered tissue. The bioreactor was built from off the shelf components, modified off the shelf components, and easily reproducible custom built parts to facilitate ease of setup, reproducibility and experimental flexibility. A T-flask was modified to allow for four tissue samples, mechanical actuation via a LabView controlled stepper motor and transduction of force from inside the T-flask to an external sensor. In vitro bench top testing with instrumentation springs and tissue culture experiments were performed to validate system performance. Force sensors were highly linear (R~2 > 0.998) and able to maintain force readings for extended periods of time. Tissue culture experiments involved cyclic loading of polyur-ethane scaffolds seeded with and without (control) human foreskin fibroblasts for 8 h/day for 14 days. After supplementation with TGF-β, tissue constructs showed an increase in stiffness between consecutive days and from the acellular controls. These experiments confirmed the ability of the bioreactor to distinguish experimental groups and monitor tissue stiffness during tissue development.
机译：控制环境条件（例如机械刺激）对于将细胞导入功能组织至关重要。这项研究报告了一种能够控制机械环境并连续测量工程组织中力位移的生物反应器的开发。该生物反应器是从现成的组件中构建，修改后的现成组件以及易于复制的定制部件，以简化设置，可复制性和实验灵活性。修改了一个T形烧瓶，以允许采集四个组织样本，通过LabView控制的步进电机进行机械致动，并将力从T形烧瓶内部传递到外部传感器。用仪器弹簧进行体外台式测试和组织培养实验以验证系统性能。力传感器是高度线性的（R〜2> 0.998），能够长时间保持力读数。组织培养实验涉及循环接种负载有和没有（对照）人包皮成纤维细胞的聚氨酯支架，每天8小时，共14天。补充TGF-β后，组织构建物在连续数天之间和从无细胞对照组显示出刚度增加。这些实验证实了生物反应器在组织发育过程中区分实验组和监测组织硬度的能力。

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