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Gossypium arboreum GHSP26 Enhances Drought Tolerance in Gossypium Hirsutum

机译:陆地棉GHSP26增强了棉球菌的耐旱性

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Heat-shock proteins (HSP) are molecular chaperones for protein molecules. These proteins play an important role in protein-protein interactions such as, folding and assisting in the establishment of proper protein conformation and prevention of unwanted protein aggregation. A small HSP gene GHSP26 present in Gossypium arboreum responds to dehydration. In the present study, an attempt was made to overcome the problem of drought stress in cotton. A cDNA of GHSP26 was isolated from G. arboreum, cloned in plant expression vector, pCAMBIAr-1301 driven by the cauliflower mosaic virus 35S promoter and introduced into Gossypium hirsutum. The integration and expression studies of putative transgenic plants were performed through GUS assay; PCR from genomic DNA, and quantitative real-time PCR analysis. Transgenic cotton plants showed an enhanced drought tolerance, suggesting that GHSP26 may play a role in plant responsiveness to drought.
机译:热休克蛋白(HSP)是蛋白质分子的分子伴侣。这些蛋白质在蛋白质与蛋白质的相互作用中起着重要作用,例如折叠并帮助建立适当的蛋白质构象并防止有害的蛋白质聚集。棉酚中存在一个小的HSP基因GHSP26对脱水反应。在本研究中,试图克服棉花的干旱胁迫问题。从植物中分离出GHSP26的cDNA,将其克隆到由花椰菜花叶病毒35S启动子驱动的植物表达载体pCAMBIAr-1301中,并导入陆地棉。通过GUS分析进行推定的转基因植物的整合和表达研究;从基因组DNA进行PCR,并进行实时定量PCR分析。转基因棉花植物显示出增强的干旱耐受性,表明GHSP26可能在植物对干旱的响应中起作用。

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