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首页> 外文期刊>Biotechnology Progress >Novel Technology to Provide an Enriched Therapeutic Cell Concentrate from Bone Marrow Aspirate
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Novel Technology to Provide an Enriched Therapeutic Cell Concentrate from Bone Marrow Aspirate

机译：从骨髓抽吸物中提供浓缩治疗细胞浓缩液的新技术

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Current strategies to repair fractures rely on orthopaedic surgeons harvesting bone from one area of the body, typically pelvis and transferring it to the fracture site. The amount of tissue available is therefore limited, requiring a second surgical procedure and often causing the patient long term pain. An alternative approach is utilise therapeutic cells contained within bone marrow aspirate during the primary procedure. The number of therapeutic cells within a fresh aspirate is insufficient to provide clinically acceptable bone healing in a time-scale that is satisfactory to the surgeon and the patient. Therefore methods to efficiently concentrate bone marrow in the clinical setting are required. Centrifugation is the current method of choice but has limitations in that it requires large capital equipment, servicing and there are potential issues of tissue contamination. We have developed a novel, acoustically-assisted filtration device that addresses these limitations, delivering a concentrated bone marrow in a point of care, single use, fully disposable, compact device. An additional advantage is that the level of concentration required can be specified by the end user. The resulting bone marrow concentrate has been characterised in terms of cell number, viability and osteogenic potential using flow cytometry and alkaline phosphatase assay. When compared to recent clinical studies using bone marrow to repair non-union fractures, the findings from our work suggest that the bone marrow concentrate is likely to be highly therapeutic and clinically efficacious as a bone fracture repair strategy. A product concept for use in the clinical setting is presented.
机译：当前修复骨折的策略依赖于整形外科医生从身体的一个区域（通常是骨盆）采集骨头并将其转移到骨折部位。因此，可用组织的数量是有限的，需要第二次外科手术并且经常引起患者长期疼痛。另一种方法是在主要手术过程中利用骨髓抽吸物中包含的治疗细胞。新鲜抽吸物中的治疗细胞数量不足以在外科医生和患者满意的时间范围内提供临床上可接受的骨愈合。因此，需要在临床环境中有效浓缩骨髓的方法。离心是当前的选择方法，但存在局限性，因为它需要大量的固定设备，维修服务，并且存在组织污染的潜在问题。我们已经开发出一种新型的声学辅助过滤设备，可解决这些局限性，可在护理点，一次性使用，完全一次性使用的紧凑型设备中输送浓缩的骨髓。另一个优点是最终用户可以指定所需的浓度水平。使用流式细胞仪和碱性磷酸酶测定法对所得的骨髓浓缩液进行了细胞数量，活力和成骨潜能的表征。与最近使用骨髓修复不愈合骨折的临床研究相比，我们的研究结果表明，骨髓浓缩物作为骨折修复策略可能具有很高的治疗性和临床疗效。介绍了用于临床环境的产品概念。

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