• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Purification of Human Antibodies from Transgenic Corn Using Aqueous Two-Phase Systems
【24h】

Purification of Human Antibodies from Transgenic Corn Using Aqueous Two-Phase Systems

机译：使用两相水系统从转基因玉米中纯化人源抗体

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A recombinant human antibody expressed in corn was purified using aqueous two-phase extraction. The antibody was an immunoglobulin G fully unglycosylated. Using systems of different compositions and/or pHs in each of one or two partitioning stages followed by one more stage in which the antibody was precipitated at the liquid/liquid interface facilitated the removal of different impurities in each stage. The best system yields a product 72% pure (22-fold purification) with a yield of 49%. The optimum extraction was done in two partitioning stages followed by an interfacial precipitation stage using poly(ethylenejglycoll potassium phosphate systems. NaCl was added to the first stage to eliminate large molecular weight impurities. The pH in the first stage was kept at 6 but a pH of 8 was used in the second stage and in the precipitation stage.
机译：使用水相萃取法纯化在玉米中表达的重组人抗体。该抗体是完全未糖基化的免疫球蛋白G。在一个或两个分配阶段的每一阶段中使用不同组成和/或pH值的系统，然后再进行一个阶段，其中抗体在液/液界面处沉淀，这有助于在每个阶段中除去不同的杂质。最好的系统产生的产品纯度为72％（纯化22倍），产率为49％。最佳萃取过程分为两个分配阶段，随后是使用聚乙二醇磷酸钾系统的界面沉淀阶段，在第一阶段添加NaCl以消除大分子量杂质，第一阶段的pH保持在6，但pH在第二阶段和沉淀阶段使用了八分之一。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号