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首页> 外文期刊>Biotechnology Progress >Alginate coating of Xenopus laevis embryos
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Alginate coating of Xenopus laevis embryos

机译：非洲爪蟾胚胎的藻酸盐涂层

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摘要

Xenopus laevis eggs were coated, immediately after squeeze-stripping and fertilization, with a thin layer (approx50 #mu#m) of film based on one of three different types of alginates which varied in their mannuronic/guluronic acid ratio. The alginatewas cross-linked with either Ca or Ba ions at three different concentrations. The developmental, survival, and hatching of these embryos and the swelling of their natural jelly coats or hydrocolloid coatings were studied over 7 days, while embryos weremaintained in flowing aerated water at a ratio of 85 mL per embryo or at a very diminished ratio of 1.6 mL of sterile ov nonsterile MMR solution per embryo. All experiments were conducted in triplicate at 20 +- 1 deg C. Oxygen was monitored continuously.Mineral content was determined in the alginate-jelly coat and within the embryos over time. The coating conferred major advantages when the ratio between the embryos and the surrounding medium was at a minimum under nonsterile conditions, perhaps as a result of the film's resistance to diffusion. In the studied systems, the coating seemed to postpone embryo hatching to a more developed stage. In addition, the coating served as a barrier to microbial contamination and thus improved survival prospects.
机译：在挤压和施肥后，立即将非洲爪蟾卵涂有一层薄薄的薄膜（约50μm），该薄膜是基于三种不同类型的藻酸盐（其甘露糖醛酸/古洛糖醛酸的比例不同）中的一种。藻酸盐与Ca或Ba离子以三种不同浓度交联。研究了这些胚胎的发育，存活和孵化以及它们的天然果冻涂层或水胶体涂层的溶胀历时7天，而胚胎则在流动的充气水中以每只胚胎85 mL的比例或以1.6的极小比例保持每只胚胎毫升无菌的非无菌MMR溶液。所有实验均在20±1℃下一式三份进行。连续监测氧气。测定藻酸盐-果冻皮中和胚胎中矿物质的含量。当在非无菌条件下胚胎与周围培养基之间的比例最小时，涂层可能具有主要优势，这可能是由于薄膜的抗扩散性所致。在研究的系统中，涂层似乎将胚胎孵化推迟到了更成熟的阶段。此外，该涂层还可以防止微生物污染，从而提高了生存前景。

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