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首页> 外文期刊>Biotechnology Progress >Use of Enzymes in Extraction of Polyhydroxyalkanoates Produced by Cupriavidus necator
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Use of Enzymes in Extraction of Polyhydroxyalkanoates Produced by Cupriavidus necator

机译：酶在提取铜尿杆菌产生的多羟基链烷酸酯中的用途

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摘要

Poly(3-hydroxybutyrate) (P(3HB)) and its copolymer poly(3-hydroxybutyrate-co-3-hydroxy-valerate) (P(3HB-co-3HV), are biodegradable thermoplastic polymers. They are members of the polyhydroxyalkanoate (PHA) family, synthesized and accumulated as a carbon and energy reserve by a variety of microorganisms. The aim of this study was to evaluate the use of the proteases Corolase~R L10, Alcalase~R 2.4L, Corolase~R 7089 and Protemax~R FC and glycosidases Celumax~R BC, Rohament~R CL and Rohalase" Barley for the recovery of P(3HB) and P(3HB-co-3HV) synthesized by Cupriavidus necator. The enzyme Celumax~R BC provided better lysis of the bacterial cell membrane and the results for the optimization of the operating conditions showed that this enzyme is most stable in acetate buffer at pH 4.0, bath at 60°C, hydrolysis time of I h and concentration of 0.02% (wlw). The optimization of the operating conditions showed that the enzyme Celumax~R BC provided better lysis of the bacterial cell in acetate buffer at pH 4.0, bath at 60°C, hydrolysis time of 1 h and concentration of 0.02% (wlw). These conditions resulted in lysis of the membrane of the bacteria with a recovery of 93.2% P(3HB-co-3HV) with 94% purity. The results showed that the use of enzymes for the polymer extraction is an efficient process that assists in the cell disruption of Cupriavidus necator.
机译：聚（3-羟基丁酸酯）（P（3HB））及其共聚物聚（3-羟基丁酸酯-co-3-羟基-戊酸酯）（P（3HB-co-3HV）是可生物降解的热塑性聚合物。它们是聚羟基链烷酸酯的成员（PHA）家族，通过多种微生物合成并作为碳和能量储备积累，旨在评估蛋白酶Corolase〜R L10，Alcalase〜R 2.4L，Corolase〜R 7089和Protemax的用途〜R FC和糖苷酶Celumax〜R BC，Rohament〜R CL和Rohalase“大麦，用于回收Cupriavidus合成的P（3HB）和P（3HB-co-3HV）。酶Celumax〜R BC可以更好地裂解细菌细胞膜和优化操作条件的结果表明，该酶在pH 4.0的乙酸盐缓冲液，60°C的浴液，1 h的水解时间和0.02％（wlw）的浓度下最稳定。的操作条件表明，Celumax〜R BC酶可以更好地溶解醋酸纤维素中的细菌细胞。在pH 4.0下耐酸性，在60°C下浴，水解时间为1 h，浓度为0.02％（wlw）。这些条件导致了细菌膜的裂解，回收了93.2％的P（3HB-co-3HV），纯度为94％。结果表明，将酶用于聚合物提取是一种有效的过程，可帮助破坏铜杯霉菌的细胞。

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