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首页> 外文期刊>Biotechnology Progress >Computational Fluid Modeling and Performance Analysis of a Bidirectional Rotating Perfusion Culture System
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Computational Fluid Modeling and Performance Analysis of a Bidirectional Rotating Perfusion Culture System

机译：双向旋转灌注培养系统的计算流体建模和性能分析

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A myriad of bioreactor configurations have been investigated as extracorporeal medical support systems for temporary replacement of vital organ functions. In recent years, studies have demonstrated that the rotating bioreactors have the potential to be utilized as bioartifi-cial liver assist devices (BLADs) owing to their advantage of ease of scalability of cell-culture volume. However, the fluid movement in the rotating chamber will expose the suspended cells to unwanted flow structures with abnormally high shear conditions that may result in poor cell stability and in turn lower the efficacy of the bioreactor system. In this study, we compared the hydrodynamic performance of our modified rotating bioreactor design with that of an existing rotating bioreactor design. Computational fluid dynamic analysis coupled with experimental results were employed in the optimization process for the development of the modified bioreactor design. Our simulation results showed that the modified bioreactor had lower fluid induced shear stresses and more uniform flow conditions within its rotating chamber than the conventional design. Experimental results revealed that the cells within the modified bioreactor also exhibited better cell-carrier attachment, higher metabolic activity, and cell viability compared to those in the conventional design. In conclusion, this study was able to provide important insights into the flow physics within the rotating bioreactors, and help enhanced the hydrodynamic performance of an existing rotating bioreactor for BLAD applications.
机译：作为用于暂时替代重要器官功能的体外医疗支持系统，已经研究了多种生物反应器配置。近年来，研究表明旋转式生物反应器具有易于扩展细胞培养体积的优势，因此有潜力用作生物人工肝辅助设备（BLAD）。然而，在旋转室内的流体运动将使悬浮细胞暴露于具有异常高剪切条件的不希望的流动结构，这可能导致较差的细胞稳定性，进而降低生物反应器系统的功效。在这项研究中，我们将改进的旋转生物反应器设计与现有旋转生物反应器设计的水动力性能进行了比较。在优化过程中，将计算流体动力学分析与实验结果相结合，用于改进生物反应器设计的开发。我们的模拟结果表明，与常规设计相比，改进的生物反应器在其旋转室内具有更低的流体感应剪切应力和更均匀的流动条件。实验结果表明，与传统设计相比，改性生物反应器中的细胞还表现出更好的细胞载体附着，更高的代谢活性和细胞活力。总之，这项研究能够为旋转生物反应器内的流动物理学提供重要见解，并有助于增强现有旋转生物反应器在BLAD应用中的流体力学性能。

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