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首页> 外文期刊>Biotechnology Progress >Rapid Removal of Glycerol from Frozen-Thawed Red Blood Cells
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Rapid Removal of Glycerol from Frozen-Thawed Red Blood Cells

机译：从冰冻融化的红细胞中快速去除甘油

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摘要

The storage of red blood cells (RBCs) in a refrigerated state allows a shelf life of a few weeks, whereas RBCs frozen in 40% glycerol have a shelf life of 10 years. Despite the clear logistical advantages of frozen blood, it is not widely used in transfusion medicine. One of the main reasons is that existing post-thaw washing methods to remove glycerol are prohibitively time consuming, requiring about an hour to remove glycerol from a single unit of blood. In this study, we have investigated the potential for more rapid removal of glycerol. Using published biophysical data for human RBCs, we mathematically optimized a three-step deglycerolization process, yielding a procedure that was less than 32 s long. This procedure was found to yield 70% hemolysis, a value that was much higher than expected. Consequently, we systematically evaluated three-step deglycerolization procedures, varying the solution composition and equilibration time in each step. Our best results consisted of less than 20% hemolysis for a deglycerolization time of 3 min, and it is expected that even further improvements could be made with a more thorough optimization and more reliable biophysical data. Our results demonstrate the potential for significantly reducing the deglycerolization time compared with existing methods.
机译：冷藏状态下的红细胞（RBC）的保存期为数周，而冷冻在40％甘油中的RBC的保存期为10年。尽管冷冻血液具有明显的后勤优势，但并未在输血医学中广泛使用。主要原因之一是，现有的解冻后洗涤方法去除甘油非常耗时，需要大约一个小时才能从单个血液中去除甘油。在这项研究中，我们研究了更快速去除甘油的潜力。使用已发布的人类RBC的生物物理数据，我们在数学上优化了三步除糖过程，产生的过程少于32 s。发现该程序可产生70％的溶血，该值远高于预期。因此，我们系统地评估了三步脱甘油步骤，改变了每一步的溶液组成和平衡时间。我们最好的结果是在3分钟的去甘油化时间内溶血率低于20％，并且有望通过更彻底的优化和更可靠的生物物理数据来进一步改善。我们的结果表明，与现有方法相比，可显着减少脱甘油时间。

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