• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Preparation of a Novel Hydrophobic Affinity Cryogel for Adsorption of Lipase and Its Utilization as a Chromatographic Adsorbent for Fast Protein Liquid Chromatography
【24h】

Preparation of a Novel Hydrophobic Affinity Cryogel for Adsorption of Lipase and Its Utilization as a Chromatographic Adsorbent for Fast Protein Liquid Chromatography

机译：新型疏水亲和力低温凝胶的制备及其对脂肪酶的吸附及其在快速蛋白质液相色谱中的色谱吸附作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, we have prepared a hydrophobic cryogel for the chromatographic separation of lipase from its aqueous solutions including single protein and protein mixture and also Yarrowia lipolytica cell extract. N-methacryloyl-(L)-phenylalanine methyl ester was used as a monomer to provide the hydrophobic character to the prepared cryogels. The highest adsorption capacity was observed at pH 5.0 at 0.5 mL min~(-1) flow rate. The chromatographic separation of lipase was achieved from a binary mixture of lipase:bovine serum albumin (BSA) and lipase.iysozyme, and was also achieved from triple-mixture of lipase dy-sozyme:BSA by using fast protein liquid chromatography. Finally, lipase purification was performed from Yarrowia lipolytica cell extract used as a natural source. These studies have shown that the hydrophobic cryogel has good chromatographic performance for the separation and purification of lipase not only from aqueous solution, but also from cell extract as a natural source of lipase.
机译：在这项研究中，我们制备了疏水性冷冻凝胶，用于从水溶液中分离脂肪酶，包括单一蛋白质和蛋白质混合物以及解脂耶氏酵母细胞提取物。 N-甲基丙烯酰基-（L）-苯丙氨酸甲酯用作单体，为制得的冰凝胶提供疏水特性。在pH 5.0和0.5 mL min〜（-1）流速下观察到最高的吸附容量。从脂肪酶：牛血清白蛋白（BSA）和脂肪酶同工酶的二元混合物中分离脂肪酶，也使用快速蛋白质液相色谱法从脂肪酶dy-sozyme：BSA的三重混合物中分离出来。最后，从用作天然来源的解脂耶氏酵母细胞提取物中进行脂肪酶纯化。这些研究表明，疏水性冷冻凝胶不仅从水溶液中分离脂肪酶，而且从天然脂肪酶的细胞提取物中分离纯化脂肪酶，也具有良好的色谱性能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号