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首页> 外文期刊>Alcohol >Histiotypic electrophysiological responses of cultured neuronal networks to ethanol.
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Histiotypic electrophysiological responses of cultured neuronal networks to ethanol.

机译：培养的神经元网络对乙醇的组织型电生理反应。

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Embryonic murine neuronal networks cultured on substrate-integrated microelectrode arrays were used to quantify acute electrophysiological effects of ethanol by using extracellular, multichannel recording of action potentials. Spontaneously active frontal cortex cultures showed repeatable, concentration-dependent sensitivities to ethanol, with initial inhibition at 20 mM and a spike rate 50% effective concentration (EC(50)) of 48.8+/-5.4 mM. Ethanol concentrations of greater than 100 mM led to cessation of activity. The ethanol inhibitions up to the maximum tested 160 mM were reversible. Although ethanol did not change the shape of action potentials, unit-specific spike pattern effects were found. At 40 mM, ethanol decreased neuronal firing in 71%, increased firing in 20%, and generated no effect in 9% of all units observed (14 cultures, 200 discriminated units). The effects of combined application of ethanol and fluoxetine were additive. Excellent agreement with findings obtained from experimental studies with animals validates the use of these in vitro systems for alcohol research.
机译：培养在底物集成微电极阵列上的胚胎鼠神经元网络被用来通过使用细胞外，多通道动作电位记录来量化乙醇的急性电生理作用。自发活跃的额叶皮层培养物对乙醇具有可重复的，浓度依赖性的敏感性，在20 mM时具有初始抑制作用，峰值速率50％有效浓度（EC（50））为48.8 +/- 5.4 mM。乙醇浓度大于100 mM导致活性停止。直至最大测试的160 mM的乙醇抑制作用是可逆的。尽管乙醇不会改变动作电位的形状，但发现了单位特异性的尖峰模式效应。在40 mM时，乙醇降低了71％的神经元放电，增加了20％的放电，并且在观察到的所有单位的9％中没有产生作用（14种培养物，200个可区分的单位）。乙醇和氟西汀联合应用的效果是相加的。与动物实验研究获得的结果极好的一致性，证实了这些体外系统用于酒精研究的有效性。

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