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首页> 外文期刊>Alcohol >Changes in gene expression in regions of the extended amygdala of alcohol-preferring rats after binge-like alcohol drinking.
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Changes in gene expression in regions of the extended amygdala of alcohol-preferring rats after binge-like alcohol drinking.

机译：酗酒样饮酒后嗜酒精的大鼠延伸杏仁核区域基因表达的变化。

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The objective of this study was to determine time-course changes in gene expression within two regions of the extended amygdala after binge-like alcohol drinking by alcohol-preferring (P) rats. Adult male P rats were given 1-h access to 15 and 30% ethanol three times daily for 8 weeks. Rats (n = 10/time point for ethanol and n = 6/time point for water) were killed by decapitation 1, 6, and 24 h after the last drinking episode. RNA was prepared from individual micropunch samples of the nucleus accumbens shell (ACB-shell) and central nucleus of the amygdala (CeA); analyses were conducted with Affymetrix Rat Genome 230.2 GeneChips. Ethanol intakes were 1.5-2 g/kg for each of the three sessions. There were no genes that were statistically different between the ethanol and water control groups at any individual time point. Therefore, an overall effect, comparing the water control and ethanol groups, was determined. In the ACB-shell and CeA, there were 276 and 402 probe sets for named genes, respectively, that differed between the two groups. There were 1.5-3.6-fold more genes with increased expression than with decreased expression in the ethanol-drinking group, with most differences between 1.1- and 1.2-fold. Among the differences between the ethanol and water control groups were several significant biological processes categories that were in common between the two regions (e.g., synaptic transmission, neurite development); however, within these categories, there were few genes in common between the two regions. Overall, the results indicate that binge-like alcohol drinking by P rats produces region-dependent changes in the expression of genes that could alter transcription, synaptic function, and neuronal plasticity in the ACB-shell and CeA; within each region, different mechanisms may underlie these alterations because there were few common ethanol-responsive genes between the ACB-shell and CeA.
机译：这项研究的目的是确定偏好酒精（P）的大鼠暴饮暴饮后，杏仁核的两个区域内基因表达的时程变化。成年雄性P大鼠每天3次每天1次获得15％和30％乙醇，持续8周。在最后一次饮酒后的第1、6和24小时，断头处死大鼠（n = 10 /乙醇时间点，n = 6 /水时间点）。从伏隔核壳（ACB-壳）和杏仁核中央核（CeA）的单个微穿孔样品中制备RNA;使用Affymetrix大鼠基因组230.2基因芯片进行分析。在这三个阶段中，每次乙醇摄入量为1.5-2 g / kg。在任何单个时间点，乙醇对照组和水对照组之间均没有统计学上差异的基因。因此，确定了比较水控制和乙醇基团的总体效果。在ACB-shell和CeA中，分别有276个和402个命名基因的探针组，两组之间存在差异。在酒精饮料组中，表达增加的基因比表达减少的基因多1.5-3.6倍，差异最大的是1.1-1.2倍。乙醇对照组和水对照组之间的差异是两个区域之间共有的几个重要的生物过程类别（例如，突触传递，神经突发育）;但是，在这些类别中，两个区域之间几乎没有共同的基因。总体而言，结果表明，P鼠酗酒样饮酒会导致基因表达的区域依赖性变化，从而可能改变ACB壳和CeA中的转录，突触功能和神经元可塑性。在每个区域内，不同的机制可能是这些变化的基础，因为ACB壳和CeA之间几乎没有常见的乙醇反应基因。

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